Microbial Strain Collection, Helmholtz Center for Infection Research, Inhoffenstraße 7, 38124, Brunswick, Germany.
Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Cibinong, 16911, Indonesia.
Antonie Van Leeuwenhoek. 2022 Aug;115(8):1059-1072. doi: 10.1007/s10482-022-01756-2. Epub 2022 Jun 22.
Four novel strains were isolated: PWU4 and PWU20 were both from soil in Germany, PWU5 was isolated from soil in India and PWU37 was obtained from sheep faeces collected on the Island of Crete. Cells of each were observed to be Gram-negative, strictly aerobic, rod shaped, and to grow optimally between 28 and 34 °C, between pH 7.0 and 8.0 and without the addition of NaCl. The strains were found to be catalase and oxidase-negative and able to grow on most mono- and disaccharides, a few polysaccharides and organic acids. Their predominant menaquinone was identified as MK-7. Their major fatty acids were identified as C ω7c (PWU4 and PWU20) and C ω5c (PWU5 and PWU37). The DNA G + C contents of strains PWU4, PWU20, PWU5 and PWU37 were determined to be 50.2 mol%, 51.6 mol %, 39.8 mol% and 53.8 mol%, respectively. The 16S rRNA gene sequence analysis revealed that the close relatives Ohtaekwangia koreensis 3B-2 and Ohtaekwangia kribbensis 10AO share less than 93.8% sequence similarity. The strains were classified in two groups, where PWU4 and PWU20 share 93.0% sequence similarity, and PWU5 and PWU37 share 97.5% sequence similarity. However, the members of each group were concluded to represent different species based on the low average nucleotide identity (ANI) of their genomes, 69.7% and 83.8%, respectively. We propose that the four strains represent four novel species of two new genera in the family Cytophagaceae. The type species of the novel genus Chryseosolibacter is Chryseosolibacter histidini gen. nov., sp. nov. with the type strain PWU4 (= DSM 111594 = NCCB 100798), whilst strain PWU20 (= DSM 111597 = NCCB 100800) is the type strain of a second species, Chryseosolibacter indicus sp. nov. The type species of the novel genus Dawidia is Dawidia cretensis gen. nov., sp. nov. with the type strain PWU5 (= DSM 111596 = NCCB 100799), whilst strain PWU37 (= DSM 111595 = NCCB 100801) is the type stain of a second species, Dawidia soli sp. nov.
从德国土壤中分离到的 PWU4 和 PWU20、从印度土壤中分离到的 PWU5 以及从克里特岛绵羊粪便中分离到的 PWU37 这四个新菌株均为革兰氏阴性、严格需氧、杆状、最佳生长温度为 28 至 34°C、pH 值为 7.0 至 8.0 且无需添加 NaCl。这些菌株均为过氧化氢酶和氧化酶阴性，可以在大多数单糖和二糖、少数多糖和有机酸上生长。它们的主要menaquinone 被鉴定为 MK-7。其主要脂肪酸被鉴定为 C ω7c（PWU4 和 PWU20）和 C ω5c（PWU5 和 PWU37）。菌株 PWU4、PWU20、PWU5 和 PWU37 的 DNA G+C 含量分别为 50.2 mol%、51.6 mol%、39.8 mol%和 53.8 mol%。16S rRNA 基因序列分析表明，密切相关的 Ohtaekwangia koreensis 3B-2 和 Ohtaekwangia kribbensis 10AO 的序列相似性小于 93.8%。这些菌株被分为两组，其中 PWU4 和 PWU20 的序列相似性为 93.0%，PWU5 和 PWU37 的序列相似性为 97.5%。然而，每组的成员都被认为代表不同的物种，因为它们的基因组的平均核苷酸同一性（ANI）较低，分别为 69.7%和 83.8%。我们建议这四个菌株代表细胞色素科的两个新属中的四个新种。新型属 Chryseosolibacter 的模式种是 Chryseosolibacter histidini gen. nov.，sp. nov.，其模式菌株为 PWU4（=DSM 111594=NCCB 100798），而菌株 PWU20（=DSM 111597=NCCB 100800）是第二种物种 Chryseosolibacter indicus sp. nov.的模式菌株。新型属 Dawidia 的模式种是 Dawidia cretensis gen. nov.，sp. nov.，其模式菌株为 PWU5（=DSM 111596=NCCB 100799），而菌株 PWU37（=DSM 111595=NCCB 100801）是第二种物种 Dawidia soli sp. nov.的模式菌株。
