Cox J C, Rudolph P, Ellington A D
Department of Chemistry and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712-1095, USA.
Biotechnol Prog. 1998 Nov-Dec;14(6):845-50. doi: 10.1021/bp980097h.
In vitro selection can be used to generate nucleic acid ligands (aptamers) to target molecules ranging in size and structure from cations to cells. However, the selection process is repetitive and time-consuming. We have automated a protocol for in vitro selection using an augmented Beckman Biomek 2000 pipetting robot. The automated selection procedure requires the integration of four devices and the optimization of four molecular biology methods, and is one of the most complex automated protocols attempted to date. Initial attempts at selection yielded robust replication parasites, but optimization of the automated selection procedure suppressed the emergence of these parasites and led to the selection of true nucleic acid ligands. Automated selection can now be used to generate nucleic acid aptamers in days rather than weeks or months.
体外筛选可用于生成核酸配体（适配体），以靶向从阳离子到细胞等大小和结构各异的分子。然而，筛选过程既重复又耗时。我们利用一台增强型贝克曼Biomek 2000移液机器人实现了体外筛选方案的自动化。自动化筛选程序需要整合四种设备并优化四种分子生物学方法，是迄今为止尝试过的最复杂的自动化方案之一。最初的筛选尝试产生了大量复制性寄生虫，但自动化筛选程序的优化抑制了这些寄生虫的出现，并导致了真正核酸配体的筛选。现在，自动化筛选可在数天而非数周或数月内用于生成核酸适配体。
