Schmidt H, Geitz C, Tarr P I, Frosch M, Karch H
Institut für Hygiene und Mikrobiologie der Universität Würzburg, Germany.
J Infect Dis. 1999 Jan;179(1):115-23. doi: 10.1086/314537.
The virulence profiles of most non-O157 Shiga toxin (Stx)-producing Escherichia coli (STEC) are unknown. STEC belonging to serogroups O26, O103, and O111 were characterized to determine clonality and to profile virulence traits. Isolates within STEC serogroups O26, O111, and O103 were >80% identical in their randomly amplified polymorphic DNA pattern, suggesting clonality in these serogroups. The serogroups were distantly related to each other and to E. coli O157:H7. stx restriction fragment length polymorphism differentiated some STEC with the same randomly amplified polymorphic DNA pattern. The hemolytic phenotype of STEC O103:H2 isolates resembled that of alpha- and not enterohemorrhagic E. coli hemolysin. Virulence gene (eae, E-hly, espP, etp, katP, stx) probing demonstrated serogroup-specific profiles. Pathogenic STEC O26, O103, and O111 belong to their own lineages and have unique profiles of virulence traits that are different from the virulence profile of E. coli O157:H7.
大多数产志贺毒素（Stx）的非O157大肠杆菌（STEC）的毒力特征尚不清楚。对属于O26、O103和O111血清型的STEC进行了特征分析，以确定其克隆性并描绘毒力特征。O26、O111和O103血清型内的分离株在随机扩增多态性DNA模式上>80%相同，表明这些血清型具有克隆性。这些血清型彼此之间以及与大肠杆菌O157:H7的亲缘关系较远。stx限制性片段长度多态性区分了一些具有相同随机扩增多态性DNA模式的STEC。STEC O103:H2分离株的溶血表型类似于α溶血而非肠出血性大肠杆菌溶血素。毒力基因（eae、E-hly、espP、etp、katP、stx）检测显示了血清型特异性特征。致病性STEC O26、O103和O111属于它们自己的谱系，并且具有与大肠杆菌O157:H7的毒力特征不同的独特毒力特征谱。
