Oropesa Fernández S, Rodríguez Peralta D, Goyenechea Hernández A, Morier Díaz L, Hernández Espinosa B, Valdivia Romero A, Chacón D, González Medina Z
Instituto de Medicina Tropical Pedro Kourí.
Rev Cubana Med Trop. 1998;50(1):36-41.
One hundred and fourty eight samples from patients with a symptomatology compatible with the influenza virus were studied aimed at identifying in a fast way these viruses. A rapid MDCK-L cell culture was developed on 96 well plates, where nasopharingeal exudates or gargarisms were inoculated and incubated all night long at 37 degrees C. The medium was removed and cells were washed with PBS and fixed with methanol. Viral antigens were detected through the immunoperoxidase staining by using two monoclonal antibody pools for the identification of influenza A and influenza B viruses. The HA1-71 monoclonal antibody, specific for influenza A (H3N2) and the HA2-76, which react with both A (H3N2) and A (H1N1) were used for subtyping. Of all the positive samples (136), 72% corresponded to type A while 34.6% and 37.5% corresponded to subtypes H1 and H3, respectively. Influenza B was detected in 27.9% of the 148 samples studied. Only 12 were negative (8.1%). The use of this technique is recommended as a rapid, convenient and sensitive method that is easy to carry put and to interpretate for the detection and characterization in type and subtype of the influenza viruses starting from the nasopharyngeal exudates or gargarisms.
对148例有与流感病毒相符症状的患者样本进行了研究，旨在快速鉴定这些病毒。在96孔板上建立了快速MDCK-L细胞培养，接种鼻咽渗出物或含漱液，并在37℃下孵育整夜。去除培养基，用PBS洗涤细胞并用甲醇固定。通过免疫过氧化物酶染色，使用两个单克隆抗体池检测病毒抗原，以鉴定甲型和乙型流感病毒。用于亚型分型的是对甲型（H3N2）特异的HA1-71单克隆抗体以及与甲型（H3N2）和甲型（H1N1）均反应的HA2-76。在所有阳性样本（136份）中，72%为甲型，而分别有34.6%和37.5%对应于H1和H3亚型。在所研究的148份样本中，27.9%检测到乙型流感。仅12份为阴性（8.1%）。推荐使用该技术作为一种快速、便捷且灵敏的方法，该方法易于实施和解读，可从鼻咽渗出物或含漱液中检测流感病毒并对其进行型别和亚型鉴定。
