[病毒性脑膜脑炎暴发。用于病毒分离的两种生物学系统的比较]
[Outbreak of viral meningoencephalitis. Comparison of 2 biological systems used for isolation of the virus].
作者信息
Abreu Nicot I M, Bello Corredor M, Más Lago P, Arteaga Hernández E, Avalos Redón I, Sarmiento Pérez L R, Palomera Puente R, Pérez Santos L
机构信息
Instituto de Medicina Tropical Pedro Kourí.
出版信息
Rev Cubana Med Trop. 1998;50(1):71-4.
An increase of the cases of viral meningoencephalitis was reported in our country from October to December, 1995. 23 faeces specimens were received from 2 provinces aimed at obtaining the viral isolation by their inoculation in cell culture and in newborn mice at the same time in order to compare the sensitivity of both systems in isolation. A higher number of isolations was obtained in cell culture. The cell culture from kidney of a normal adult green African monkey (Vero) proved to be more sensitive. 18 isolations were obtained and identified as Coxsackievirus B5. An anatomical and pathological study was conducted in inoculated mice and injuries compatible with the enteroviral infection were observed.
1995年10月至12月，我国报告的病毒性脑膜脑炎病例有所增加。从2个省份收到了23份粪便标本，目的是通过将它们同时接种到细胞培养物和新生小鼠中来进行病毒分离，以便比较两种系统在分离方面的敏感性。在细胞培养中获得了更多的分离物。事实证明，来自正常成年非洲绿猴肾脏的细胞培养物（Vero细胞）更敏感。获得了18次分离物，并鉴定为柯萨奇病毒B5。对接种的小鼠进行了解剖和病理学研究，观察到了与肠道病毒感染相符的损伤。
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