巴西一种由豇豆蚜传花叶病毒（CABMV）分离株引起的海滩刀豆花叶镶嵌病。
A mosaic of beach bean (Canavalia rosea) caused by an isolate of Cowpea aphid-borne mosaic virus (CABMV) in Brazil.
作者信息
Kitajima E W, de Alcântara B K, Madureira P M, Alfenas-Zerbini P, Rezende J A M, Zerbini F M
机构信息
Departamento de Entomologia, Fitopatologia & Zoologia Agrícola, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Caixa Postal 9, Piracicaba, 13418-900, Brazil.
出版信息
Arch Virol. 2008;153(4):743-7. doi: 10.1007/s00705-008-0052-7. Epub 2008 Feb 14.
Beach bean (Canavalia rosea) plants showing mosaic symptoms were found at Massaguaçú beach, Caraguatatuba, Brazil. A potyvirus was found to be responsible for the symptoms, based on transmission assays and electron microscopy. A positive reaction in ELISA was obtained against cowpea aphid-borne mosaic (CABMV) antisera. Viral identity was confirmed by RT-PCR using specific primers to amplify part of the NIb and the entire CP coding region of the genome and the 3'NTR. Comparison of the amplified sequences with that of CABMV showed a nucleotide sequence identity of 97% for the CP coding region. Thus, the potyvirus from beach bean should be considered a CABMV isolate, referred to as CABMV-Cr.
在巴西卡拉瓜塔图巴的马萨瓜苏海滩发现了表现出花叶症状的海滨豆（Canavalia rosea）植株。基于传播试验和电子显微镜观察，发现一种马铃薯Y病毒是造成这些症状的原因。用酶联免疫吸附测定（ELISA）检测发现，该病毒与豇豆蚜传花叶病毒（CABMV）抗血清呈阳性反应。通过逆转录聚合酶链反应（RT-PCR），使用特异性引物扩增基因组的部分NIb和整个CP编码区以及3'非翻译区（3'NTR）来确认病毒的身份。将扩增序列与CABMV的序列进行比较，结果显示CP编码区的核苷酸序列同一性为97%。因此，来自海滨豆的马铃薯Y病毒应被视为CABMV的一个分离株，称为CABMV-Cr。
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