Berggren C, Bjarnason B, Johansson G
Department of Analytical Chemistry, University of Lund, Sweden.
Biosens Bioelectron. 1998 Nov 1;13(10):1061-8. doi: 10.1016/s0956-5663(98)00058-x.
An instrument for potentiostatic capacitance measurements, based on perturbation with a potentiostatic step was used. The capacitive sensor consisted of self-assembled sulfur compounds on gold to which antibodies towards Interleukine 6, Il-6, had been immobilized. The biosensor was part of a potentiostatic three-electrode system with an extra reference electrode. Two different methods using epoxy- or carbodiimide coupling of the polyclonal antibodies were compared. The antigen Il-6 could be detected from 5 x 10(-16) to 5 x 10(-13) M with one immobilization method and to more than 5 x 10(-9) M with the other. No labels were necessary since the binding of the antigen was detected directly. The insulating properties of the different layers of the biosensor were investigated.
使用了一种基于恒电位阶跃扰动的恒电位电容测量仪器。电容传感器由金上自组装的硫化合物组成，其上固定有针对白细胞介素6（Il-6）的抗体。该生物传感器是具有额外参比电极的恒电位三电极系统的一部分。比较了使用多克隆抗体的环氧偶联或碳二亚胺偶联的两种不同方法。用一种固定方法可检测到浓度范围为5×10⁻¹⁶至5×10⁻¹³ M的抗原Il-6，用另一种方法可检测到浓度超过5×10⁻⁹ M的抗原Il-6。由于直接检测到了抗原的结合，因此无需标记。研究了生物传感器不同层的绝缘性能。
