Hagberg I A, Roald H E, Lyberg T
Research Forum, Ullevaal University Hospital, Oslo, Norway.
Thromb Haemost. 1998 Nov;80(5):852-8.
Since the role of leukocytes found present in thrombi and haemostatic plugs is not clearly understood. we have investigated the interaction between leukocytes and growing thrombi in a human ex vivo model of arterial thrombogenesis. At a wall shear rate characteristic of moderately stenosed arteries (2600 s(-1)), granulocytes selectively accumulated at the luminal surface of platelet thrombi. The leukocyte adhesion seemed independent of fibrin formation and was clearly correlated to thrombus growth and platelet activation. In contrast, flow cytometry revealed that the expression of adhesion molecules (CD11a, CD11b, CD11, CD3, CD14, CD62L, HLA-DR and binding of fibrinogen) on the surface of circulating leukocytes passing the thrombi was, on short term conditions (15 min), independent of thrombus growth. The adhered granulocytes probably play a pivotal role in limiting the size of the evolving thrombi, as suggested by our electron micrographs of the arterial thrombi showing lysed and phagocytosed platelets. Thus, granulocytes might play an active role in the acute/semiacute phase of local thromboregulation.
由于目前对血栓和止血栓中存在的白细胞的作用尚不清楚，我们在人类动脉血栓形成的体外模型中研究了白细胞与正在形成的血栓之间的相互作用。在中度狭窄动脉的特征性壁剪切率（2600 s(-1)）下，粒细胞选择性地聚集在血小板血栓的管腔表面。白细胞黏附似乎与纤维蛋白形成无关，且与血栓生长和血小板活化明显相关。相比之下，流式细胞术显示，在短期条件（15分钟）下，通过血栓的循环白细胞表面黏附分子（CD11a、CD11b、CD11、CD3、CD14、CD62L、HLA-DR）的表达以及纤维蛋白原的结合与血栓生长无关。如我们对动脉血栓的电子显微镜图像所示，黏附的粒细胞可能在限制正在形成的血栓大小方面起关键作用，图像显示有溶解和被吞噬的血小板。因此，粒细胞可能在局部血栓调节的急性/亚急性期发挥积极作用。
