Weiss M T, Weninger M, Häusler J, Lubec G
University of Vienna, Department of Paediatrics.
Padiatr Padol. 1988;23(1):9-14.
3-trans-hydroxyproline is a hydroxyproline isomer present in collagens. It is more abundant in basement membrane collagen (collagen type IV) where it can be found in a relation of 12 residues per 1000 amino acid residues. This implicates its probable use as a marker substance for collagen type IV metabolism. Up to now only hydrolyzed urine samples were examined for the presence of this amino acid, thus indicating total urinary excretion. We are reporting the free urinary 3 OHP content of adults and school children. School children (age group 8-18 years) showed mean excretion of 1.066 micrograms/mg creatinine (SD +/- 0.376), SEM 0.054, min. 0.516; max. 2.397, range: 1.881. Adults (age 19-29 years) showed mean excretion of 0.618 micrograms/ml creatinine (SD +/- 0.465), SEM 0.134, min. 0.055; max. 1.634, range: 1.579. As given by the statistical calculation of unpaired t-test, adults showed significantly lower urinary 3 OHP levels: t = 3.518 and p = less than 0.0005. This can be explained by increased collagen metabolism of the growing organism and is in congruence with data reported in literature for total urinary 3 OHP excretion.
3-反式羟脯氨酸是一种存在于胶原蛋白中的羟脯氨酸异构体。它在基底膜胶原蛋白（IV型胶原蛋白）中含量更为丰富，在每1000个氨基酸残基中可发现12个该异构体残基。这意味着它可能用作IV型胶原蛋白代谢的标志物。到目前为止，仅对水解后的尿液样本进行了该氨基酸存在情况的检测，从而表明的是尿中总排泄量。我们报告了成人和学龄儿童尿液中游离3-反式羟脯氨酸（3 OHP）的含量。学龄儿童（8 - 18岁年龄组）的平均排泄量为1.066微克/毫克肌酐（标准差±0.376），标准误0.054，最小值0.516；最大值2.397，范围：1.881。成人（19 - 29岁）的平均排泄量为0.618微克/毫升肌酐（标准差±0.465），标准误0.134，最小值0.055；最大值1.634，范围：1.579。通过非配对t检验的统计计算得出，成人尿液中的3 OHP水平显著较低：t = 3.518，p < 0.0005。这可以通过生长中生物体胶原蛋白代谢增加来解释，并且与文献报道的尿中3 OHP总排泄量数据一致。
