Xiang K, Jia W, Lu H, Zheng T, Lu J, Tang J, Ding W, Sun D, Li J
Department of Endocrinology and Metabolism, Shanghai Sixth People Hospital, Shanghai 200233 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1998 Dec 10;15(6):337-40.
To ascertain the association of the Trp64Arg mutation in the beta 3-adrenergic receptor (ADRB3) gene with the total body fat and fat distribution, plasma glucose level, lipid profile, insulin secretion and action in Chinese.
Genotyping of the Trp64Arg mutation in the ADRB3 gene as well as measurment of clinical variables body mass index(BMI), percent of desirable weight(PDW), area of regional adipose tissue depots, fasting lipid profile, and also, plasma glucose, insulin, C-peptide, free fatty acids levels before and after glucose challenge and blood pressure were performed in 308 Chinese, of whom, 161 were nondiabetics and 147, type 2 diabetic subjects.
(1) The frequency of Arg64 allele of ADRB3 gene in Chinese was similar to that in Japanese but slightly higher than that in Caucasians. (2) No association of any of the clinical varaibles with Trp64Arg mutation was observed in nondiabetics. (3) The Trp64Arg mutation in the ADRB3 gene was associated with BMI(P=0.019), PDW(P=0.001) and waist circumference (P=0.045) in type 2 diabetic subjects. In female diabetic subjects, more significant association with BMI(P=0.001), PDW(P=0.001), waist circumference(P=0.023), and also, association with abdominal and femoral subcutaneous fat area(P=0.032 and P=0.023) were observed. The frequencies of Arg64 allele were 21%, 14% and 4% in female diabetics with obesity, over-weight and normal-weight respectively (P=0.026). Logistic regression analysis showed that BMI was the only independent clinical variable related to the Trp64Arg mutation in the ADRB3 gene (P=0.0027).
The Trp64Arg mutation in ADRB3 gene contributes to the variation of total body fat in Chinese type 2 diabetic subjects.
确定β3 - 肾上腺素能受体（ADRB3）基因中的色氨酸64位精氨酸（Trp64Arg）突变与中国人的全身脂肪及脂肪分布、血浆葡萄糖水平、血脂谱、胰岛素分泌和作用之间的关联。
对308名中国人进行ADRB3基因Trp64Arg突变的基因分型，并测量临床变量，包括体重指数（BMI）、理想体重百分比（PDW）、局部脂肪组织储存面积、空腹血脂谱，以及葡萄糖激发前后的血浆葡萄糖、胰岛素、C肽、游离脂肪酸水平和血压。其中，161人为非糖尿病患者，147人为2型糖尿病患者。
（1）中国人中ADRB3基因的精氨酸64等位基因频率与日本人相似，但略高于白种人。（2）在非糖尿病患者中，未观察到任何临床变量与Trp64Arg突变之间存在关联。（3）ADRB3基因中的Trp64Arg突变与2型糖尿病患者的BMI（P = 0.019）、PDW（P = 0.001）和腰围（P = 0.045）相关。在女性糖尿病患者中，观察到与BMI（P = 0.001）、PDW（P = 0.001）、腰围（P = 0.023）以及与腹部和股部皮下脂肪面积的关联更为显著（P = 0.032和P = 0.023）。肥胖、超重和体重正常的女性糖尿病患者中精氨酸64等位基因频率分别为21%、14%和4%（P = 0.026）。逻辑回归分析表明，BMI是与ADRB3基因中Trp64Arg突变相关的唯一独立临床变量（P = 0.0027）。
ADRB3基因中的Trp64Arg突变导致中国2型糖尿病患者全身脂肪发生变化。
