Sweet D, Hildebrand D
Bureau of Legal Dentistry, University of British Columbia, Vancouver, Canada.
J Forensic Sci. 1998 Nov;43(6):1199-202.
DNA has been previously recovered from human teeth for RFLP and PCR-based forensic analysis. In some cases, the maximum amount of undisturbed tooth structure is required for ulterior forensic analysis. But, in most cases, following comprehensive documentation, it is possible to section the tooth longitudinally or horizontally, or crush it to access the DNA-rich core. This technical report describes an alternative method to recover DNA from whole extracted human molar teeth. A 6700 freezer mill was used to pulverize 20 teeth under frozen preparation in liquid nitrogen and sterile conditions. The mean yield of DNA was 30.9 micrograms (18.4 micrograms DNA per gm tooth powder). The resulting fine powder was subjected to organic extraction and subsequently quantified using slot blot hybridization. Aliquots were successfully amplified at three short tandem repeat polymorphic loci. The technique is simple and relatively rapid. Isolation of the samples during pulverization minimizes the risk of contamination.
此前已从人类牙齿中提取DNA用于基于限制性片段长度多态性（RFLP）和聚合酶链反应（PCR）的法医分析。在某些情况下，后续法医分析需要最大量的未受干扰的牙齿结构。但是，在大多数情况下，经过全面记录后，可以将牙齿纵向或横向切片，或者将其碾碎以获取富含DNA的核心部分。本技术报告描述了一种从完整拔除的人类磨牙中提取DNA的替代方法。使用6700型冷冻研磨机在液氮和无菌条件下对20颗牙齿进行冷冻研磨成粉末。DNA的平均产量为30.9微克（每克牙粉含18.4微克DNA）。所得细粉进行有机提取，随后使用狭缝印迹杂交进行定量。等分试样在三个短串联重复多态性位点成功扩增。该技术简单且相对快速。研磨过程中样品的分离可将污染风险降至最低。
