Cartier R, Bouchard D
Service de Chirurgie, Institut de Cardiologie de Montréal, Québec, Canada.
Ann Chir. 1998;52(8):827-33.
Lipid peroxidation and LDL oxidation have been implicated in intimal hyperplasia and endothelial dysfunction following direct arterial trauma.
The aim of this study was to evaluate the effects of megadose of natural antioxidant vitamin E (VE) combined to vitamin C (VC) on the regenerated endothelial dysfunction.
A first group of rats (VE and C) (n = 10) was pretreated with VE (500 IU/kg/day) and VC (1200 mg/kg/day) for 4 weeks before aortic (thoracic) endothelial denudation with a Fogarty catheter. Rats were then fed with the same vitamin supplemented diet for 2 months. A second group (n = 10) was similarly denuded and treated with soya oil (SO), VE vehicle, for the same period; a third group (n = 10) was denuded only (DN); and a fourth group was maintained on a regular diet (CL) without denudation. Endothelial-dependent and independent relaxation was assessed in organ chambers.
Vascular relaxation to nitric oxide analogue sodium nitroprusside was not affected either by the regenerative process or the vitamin supplementation. However, endothelial-dependent relaxation to acetylcholine, was significantly preserved in VE and C (p < 0.01) treated animals compared to DN group.
These results suggest that, in this model, dietary megadose of antioxidants vitamin initiated 4 weeks before denudation can improve the post-regenerative endothelial dysfunction and could contribute to the prevention of the atherosclerotic process.
脂质过氧化和低密度脂蛋白氧化与直接动脉损伤后的内膜增生和内皮功能障碍有关。
本研究旨在评估大剂量天然抗氧化剂维生素E（VE）与维生素C（VC）联合使用对再生内皮功能障碍的影响。
第一组大鼠（VE和C组）（n = 10）在使用Fogarty导管进行主动脉（胸段）内皮剥脱术前4周，用VE（500 IU/kg/天）和VC（1200 mg/kg/天）进行预处理。然后，大鼠用相同的补充维生素的饮食喂养2个月。第二组（n = 10）同样进行内皮剥脱，并在同一时期用大豆油（SO）（VE的赋形剂）进行处理；第三组（n = 10）仅进行内皮剥脱（DN组）；第四组维持常规饮食（CL组），不进行内皮剥脱。在器官浴槽中评估内皮依赖性和非依赖性舒张功能。
血管对一氧化氮类似物硝普钠的舒张不受再生过程或维生素补充的影响。然而，与DN组相比，VE和C组（p < 0.01）处理的动物对乙酰胆碱的内皮依赖性舒张功能得到显著保留。
这些结果表明，在该模型中，在内皮剥脱术前4周开始给予大剂量抗氧化剂维生素饮食可改善再生后的内皮功能障碍，并可能有助于预防动脉粥样硬化进程。
