Perrey C, Pravica V, Sinnott P J, Hutchinson I V
School of Biological Sciences, University of Manchester, UK.
Transpl Immunol. 1998 Sep;6(3):193-7. doi: 10.1016/s0966-3274(98)80045-2.
Polymorphic variants of cytokine genes are associated with acute and chronic transplant rejection. In this technical report, the methods currently used in our centre to genotype individuals for interferon-gamma, interleukin-10, transforming growth factor-beta 1 and tumour necrosis factor-alpha are described in detail. The DNA sequences of primers and probes, and conditions for polymerase chain reactions are given, and the allele and genotype frequencies in our control populations are summarized.
细胞因子基因的多态性变体与急性和慢性移植排斥反应相关。在本技术报告中，详细描述了我们中心目前用于对个体进行干扰素-γ、白细胞介素-10、转化生长因子-β1和肿瘤坏死因子-α基因分型的方法。给出了引物和探针的DNA序列以及聚合酶链反应条件，并总结了我们对照人群中的等位基因和基因型频率。
