Zahedi S, Legrand R, Brunel G, Albert A, Dewé W, Coumans B, Bernard J P
Department of Periodontology and Implantology, School of Dentistry, Sart Tilman University Hospital, University of Liège, Belgium.
J Periodontol. 1998 Nov;69(11):1238-46. doi: 10.1902/jop.1998.69.11.1238.
In the present study, the potential of a diphenylphosphorylazide-crosslinked type I bovine collagen membrane was evaluated in the healing of mandibular bone defects applying the biological concept of guided bone regeneration. The experiment was carried out on 25 Wistar rats. After exposing the mandibular ramus bilaterally, 5 mm diameter full-thickness circular bone defects were surgically created. While the defect on one side was covered by the membrane (experimental), the defect on the other side was left uncovered (control) before closure of the overlying soft tissues. The rats were sacrificed in groups of 5 after 7, 15, 30, 90, and 180 days of healing. Although at early stages of healing similar amounts of bone formation were observed in the experimental and control defects, after 1 month of healing, most of the experimental defects were completely closed with new bone, while in the control defects, only limited amounts of new bone were observed at the rims and in the lingual aspect of the lesions. In the 90- and 180-day animals, all experimental defects were completely closed, while in the control defects, no statistically significant increase in bone regeneration was observed. The increase in percentage of bone regeneration in the experimental defects was statistically significant between the 15-day specimens as compared with the 7-day specimens (P < 0.01) and likewise between 30-day and 15-day specimens (P < 0.001). It can be concluded that a DPPA-crosslinked collagen membrane yields biocompatibility, ad hoc mechanical hindrance, and handling characteristics suitable for guided bone regeneration applications in this experimental model.
在本研究中，运用引导性骨再生的生物学概念，评估了二苯基磷酰叠氮交联的I型牛胶原膜在修复下颌骨缺损方面的潜力。实验在25只Wistar大鼠身上进行。双侧暴露下颌支后，通过手术制造直径5毫米的全层圆形骨缺损。在缝合覆盖的软组织之前，一侧的缺损用膜覆盖（实验组），另一侧的缺损不覆盖（对照组）。在愈合7、15、30、90和180天后，将大鼠按每组5只进行处死。尽管在愈合早期，实验组和对照组缺损处观察到相似数量的骨形成，但在愈合1个月后，大多数实验组缺损被新骨完全封闭，而在对照组缺损中，仅在缺损边缘和舌侧观察到少量新骨。在90天和180天的动物中，所有实验组缺损均完全封闭，但在对照组缺损中，未观察到骨再生有统计学意义的增加。与7天标本相比（P < 0.01），实验组缺损在第15天标本时骨再生百分比的增加具有统计学意义，同样，在30天和15天标本之间也具有统计学意义（P < 0.001）。可以得出结论，在该实验模型中DPPA交联胶原膜具有生物相容性、特定的机械屏障作用以及适合引导性骨再生应用的操作特性。
