Ahn Jong-Ju, Kim Hyung-Joon, Bae Eun-Bin, Cho Won-Tak, Choi YunJeong, Hwang Su-Hyun, Jeong Chang-Mo, Huh Jung-Bo
Department of Prosthodontics, Dental Research Institute, Dental and Life Science Institute, BK21 PLUS Project, School of Dentistry, Pusan National University, Yangsan 50612, Korea.
Department of Oral Physiology, BK21 PLUS Project, Periodontal Diseases Signaling Network Research Center, and Dental and Life Science Institute, School of Dentistry, Pusan National University, Yangsan 50611, Korea.
Materials (Basel). 2020 Oct 15;13(20):4599. doi: 10.3390/ma13204599.
The purpose of this study was to evaluate the bone regeneration efficacy of an 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked collagen membrane for guided bone regeneration (GBR). A non-cross-linked collagen membrane (Control group), and an EDC-cross-linked collagen membrane (Test group) were used in this study. In vitro, mechanical, and degradation testing and cell studies were performed. In the animal study, 36 artificial bone defects were formed in the mandibles of six beagles. Implants were inserted at the time of bone grafting, and membranes were assigned randomly. Eight weeks later, animals were sacrificed, micro-computed tomography was performed, and hematoxylin-eosin stained specimens were prepared. Physical properties (tensile strength and enzymatic degradation rate) were better in the Test group than in the Control group. No inflammation or membrane collapse was observed in either group, and bone volumes (%) in defects around implants were similar in the two groups ( > 0.05). The results of new bone areas (%) analysis also showed similar values in the two groups ( > 0.05). Therefore, it can be concluded that cross-linking the collagen membranes with EDC is the method of enhancing the physical properties (tensile strength and enzymatic degradation) of the collagen membranes without risk of toxicity.
本研究的目的是评估1-乙基-3-(3-二甲基氨基丙基)碳二亚胺(EDC)交联胶原膜用于引导骨再生(GBR)的骨再生效果。本研究使用了非交联胶原膜(对照组)和EDC交联胶原膜(试验组)。进行了体外、力学和降解测试以及细胞研究。在动物研究中，在6只比格犬的下颌骨上形成了36处人工骨缺损。在植骨时植入种植体，并随机分配使用膜。8周后，处死动物，进行微型计算机断层扫描，并制备苏木精-伊红染色标本。试验组的物理性能(拉伸强度和酶降解率)优于对照组。两组均未观察到炎症或膜塌陷，两组种植体周围缺损处的骨体积(%)相似(>0.05)。新骨面积(%)分析结果也显示两组的值相似(>0.05)。因此，可以得出结论，用EDC交联胶原膜是增强胶原膜物理性能(拉伸强度和酶降解)且无毒性风险的方法。
