Chang J, Most D, Thunder R, Mehrara B, Longaker M T, Lineaweaver W C
Division of Hand Surgery, Stanford University Medical Center, CA 94305, USA.
J Hand Surg Am. 1998 Nov;23(6):1052-8. doi: 10.1016/S0363-5023(98)80015-4.
Basic fibroblast growth factor (bFGF) is a cytokine that plays a fundamental role in angiogenesis. This study examines bFGF messenger RNA (mRNA) expression in a rabbit flexor tendon wound healing model. Thirty-four New Zealand white rabbit forepaws underwent transection and repair of the middle digit flexor digitorum profundus tendon in zone II. Tendons were harvested at increasing time intervals and analyzed by in situ hybridization and immunohistochemistry. Few tenocytes and tendon sheath cells expressed bFGF mRNA in unwounded tendons. In contrast, tendons subjected to transection and repair exhibited an increased signal for bFGF mRNA in both resident tenocytes concentrated along the epitenon and infiltrating fibroblasts and inflammatory cells from the tendon sheath. These data demonstrate that (1) normal tenocytes and tendon sheath cells are capable of bFGF production, (2) bFGF mRNA is upregulated in the tendon wound environment, and (3) the upregulation of this angiogenic cytokine occurs in tenocytes as well as in tendon sheath fibroblasts and inflammatory cells.
碱性成纤维细胞生长因子（bFGF）是一种在血管生成中起重要作用的细胞因子。本研究在兔屈肌腱伤口愈合模型中检测bFGF信使核糖核酸（mRNA）的表达。34只新西兰白兔的前爪接受了II区中指指深屈肌腱的横断和修复。在不同的时间间隔采集肌腱，通过原位杂交和免疫组织化学进行分析。在未受伤的肌腱中，很少有腱细胞和腱鞘细胞表达bFGF mRNA。相比之下，横断并修复的肌腱在沿腱外膜聚集的驻留腱细胞以及来自腱鞘的浸润成纤维细胞和炎性细胞中均显示出bFGF mRNA信号增强。这些数据表明：（1）正常腱细胞和腱鞘细胞能够产生bFGF；（2）bFGF mRNA在肌腱伤口环境中上调；（3）这种血管生成细胞因子的上调发生在腱细胞以及腱鞘成纤维细胞和炎性细胞中。
