Monstein H J, Tiveljung A, Jonasson J
Division of Clinical Microbiology, Faculty of Health Sciences, University Hospital, Linköping, Sweden.
FEMS Immunol Med Microbiol. 1998 Nov;22(3):217-24. doi: 10.1111/j.1574-695X.1998.tb01209.x.
The integrity of DNA and ribosomal RNAs in exponentially growing (bacillary) and ageing stationary phase (coccoid) cultures of Helicobacter pylori type strain CCUG 17874 was investigated. Extensive non-random fragmentation of rRNAs was observed during the conversion to the coccoid form. Beside a small proportion of full-length 16S and 23S rRNA that was always present, the majority of both 16S and 23S rRNA molecules showed distinct highly specific fragmentation patterns. The 16S rRNA fragmentation was characterised in detail by means of Northern blot and primer extension analysis. One cleavage site was located within the highly conserved U5 region (position about 920). The results could not be attributed to the presence of intervening sequences in the 16S and 23S rRNA genes.
对幽门螺杆菌标准菌株CCUG 17874处于指数生长期（杆菌状）和衰老稳定期（球菌状）培养物中的DNA和核糖体RNA的完整性进行了研究。在向球菌形态转化过程中观察到rRNA广泛的非随机片段化。除了始终存在的一小部分全长16S和23S rRNA外，大多数16S和23S rRNA分子都呈现出独特的高度特异性片段化模式。通过Northern印迹和引物延伸分析对16S rRNA片段化进行了详细表征。一个切割位点位于高度保守的U5区域内（位置约为920）。结果不能归因于16S和23S rRNA基因中存在间隔序列。
