大鼠蛋白磷酸酶2A 55 kDa B调节亚基同种型的Bra、Brb和Brg基因的连锁图谱。
Linkage mapping of the Bra, Brb and Brg genes for rat protein phosphatase 2A 55 kDa B-regulatory subunit isotypes.
作者信息
Yamada S, Shima H, Toyota M, Ushijima T, Kuramoto T, Serikawa T, Okada K, Sato K, Sugimura T, Nagao M, Nakagama H
机构信息
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo.
出版信息
Jpn J Cancer Res. 1998 Oct;89(10):1014-9. doi: 10.1111/j.1349-7006.1998.tb00490.x.
We previously identified the rat Bra, Brb and Brg genes, which encode alpha, beta and gamma isotypes of the 55 kDa B-regulatory subunit of protein phosphatase 2A. Polymerase chain reaction-single strand conformation polymorphism analysis in the present study identified polymorphisms in Bra, Brb and Brg between the ACI and BUF, ZI and TM, and BN and WTC strains, respectively. Linkage analysis using mapping panels composed of F2 or back-crosses of these strains allowed Bra, Brb and Brg to be assigned to chromosomes 15, 18 and 14, respectively. Furthermore, it was revealed that Bra is located close to the Rb1 locus. Using polymorphism in Bra, loss of heterozygosity (LOH) was analyzed for rat mammary tumors induced in (SD x F344) F1 female rats by a food-borne carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, and a typical mammary carcinogen, 7,12-dimethylbenz[a]anthracene. No LOH was detected at the Bra locus.
我们之前鉴定出大鼠的Bra、Brb和Brg基因，它们编码蛋白磷酸酶2A的55 kDa B调节亚基的α、β和γ同种型。本研究中的聚合酶链反应-单链构象多态性分析分别在ACI与BUF、ZI与TM以及BN与WTC品系之间鉴定出了Bra、Brb和Brg中的多态性。使用由这些品系的F2或回交组成的定位板进行连锁分析，结果表明Bra、Brb和Brg分别定位于15号、18号和14号染色体。此外，还发现Bra位于Rb1基因座附近。利用Bra中的多态性，对经食源性致癌物2-氨基-1-甲基-6-苯基咪唑[4,5-b]吡啶和典型的乳腺致癌物7,12-二甲基苯并[a]蒽诱导的(SD×F344)F1雌性大鼠乳腺肿瘤进行了杂合性缺失(LOH)分析。在Bra基因座未检测到LOH。
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