九个通过聚合酶链反应技术鉴定的多态性标记在大鼠8号染色体上形成了两个连锁群。

Nine polymorphic markers characterized by polymerase chain reaction techniques form two linkage groups on rat chromosome 8.

作者信息

Mathern P, Goldmuntz E A, Du Y, Zha H, Cash J M, Crofford L J, Wilder R L, Remmers E F

机构信息

Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.

出版信息

Cytogenet Cell Genet. 1994;66(4):283-6. doi: 10.1159/000133713.

DOI:10.1159/000133713

PMID:7909284

Abstract

Five genes and four anonymous polymorphic markers, forming two linkage groups, were mapped in F2 intercross progeny of F344/N x LEW/N rats using polymerase chain reaction (PCR) techniques. Both linkage groups were assigned to rat chromosome 8 because they contained genetic loci previously mapped to this chromosome. The first group was comprised of markers for three anonymous loci and two gene loci, thymus cell antigen-1 (Thy1) and tropoelastin (Eln). The second group was comprised of markers for one anonymous locus and three gene loci, cellular retinol binding protein II (Rbp2), matrin F/G (Matr1), and acyl-peptide hydrolase (Apeh). Seven markers (identified by simple sequence repeat associated length polymorphisms) were characterized in an additional 13 inbred rat strains (ACI/N, BN/SsN, BUF/N, LER/N, LOU/MN, MNR/N, MR/N, SHR/N, SR/Jr, SS/Jr, WBB1/N, WBB2/N, and WKY/N). Two to six alleles were detected for each marker. The reported markers should facilitate genetic mapping and monitoring of inbred rat strains.

摘要

利用聚合酶链反应（PCR）技术，在F344/N×LEW/N大鼠的F2杂交后代中对五个基因和四个匿名多态性标记进行了定位，这些标记形成了两个连锁群。由于这两个连锁群都包含先前定位到大鼠8号染色体上的遗传位点，因此它们都被定位到了大鼠8号染色体上。第一组由三个匿名位点以及两个基因位点——胸腺细胞抗原-1（Thy1）和原弹性蛋白（Eln）的标记组成。第二组由一个匿名位点以及三个基因位点——细胞视黄醇结合蛋白II（Rbp2）、核基质蛋白F/G（Matr1）和酰基肽水解酶（Apeh）的标记组成。在另外13个近交系大鼠品系（ACI/N、BN/SsN、BUF/N、LER/N、LOU/MN、MNR/N、MR/N、SHR/N、SR/Jr、SS/Jr、WBB1/N、WBB2/N和WKY/N）中对七个标记（通过简单序列重复相关长度多态性鉴定）进行了特征分析。每个标记检测到2至6个等位基因。所报道的这些标记应该有助于近交系大鼠品系的遗传图谱绘制和监测。