真核起始因子3（eIF3）复合体亚基的拟南芥同源物与COP9复合体相关联。

The Arabidopsis homologue of an eIF3 complex subunit associates with the COP9 complex.

作者信息

Karniol B, Yahalom A, Kwok S, Tsuge T, Matsui M, Deng X W, Chamovitz D A

机构信息

Department of Plant Sciences, Tel Aviv University, Israel.

出版信息

FEBS Lett. 1998 Nov 13;439(1-2):173-9. doi: 10.1016/s0014-5793(98)01367-2.

DOI:10.1016/s0014-5793(98)01367-2

PMID:9849901

Abstract

The Arabidopsis COP9 complex is a multi-subunit repressor of photomorphogenesis which is conserved among multicellular organisms. Approximately 12 proteins copurify with the COP9 complex. Seven of these proteins are orthologues of subunits of the recently published mammalian COP9 complex. Four of the proteins show amino acid similarity to various subunits of the COP9 complex, eIF3 complex and 19S cap of the proteasome. We have studied one of these proteins in order to determine if it is a component of the COP9 complex. Arabidopsis p105 is highly similar to the p110 subunit of the human elF3. The p105 gene is induced during photomorphogenesis, and RNA and protein analysis reveal different tissue accumulation patterns. p105 is found in a large protein complex. p105 interacts in yeast with both COP9 and FUS6, two known components of the COP9 complex. Our results indicate that p105 is not a component of the COP9 core complex, though it may interact with components of the complex.

摘要

拟南芥COP9复合体是光形态建成的多亚基阻遏物，在多细胞生物中保守。约有12种蛋白质与COP9复合体共纯化。其中7种蛋白质是最近发表的哺乳动物COP9复合体亚基的直系同源物。4种蛋白质与COP9复合体、eIF3复合体和蛋白酶体19S帽的各种亚基显示氨基酸相似性。我们研究了其中一种蛋白质，以确定它是否是COP9复合体的一个组分。拟南芥p105与人类eIF3的p110亚基高度相似。p105基因在光形态建成过程中被诱导，RNA和蛋白质分析揭示了不同的组织积累模式。p105存在于一个大的蛋白质复合体中。p105在酵母中与COP9复合体的两个已知组分COP9和FUS6相互作用。我们的结果表明，p105不是COP9核心复合体的一个组分，尽管它可能与该复合体的组分相互作用。