卡波西肉瘤中的人类疱疹病毒8型（HHV - 8）：与Bcl - 2和p53蛋白表达缺乏关联。
Human herpes virus 8 (HHV-8) in Kaposi's sarcoma: lack of association with Bcl-2 and p53 protein expression.
作者信息
Kennedy M M, O'Leary J J, Oates J L, Lucas S B, Howells D D, Picton S, McGee J O
机构信息
Nuffield Department of Pathology and Bacteriology, University of Oxford, UK.
出版信息
Mol Pathol. 1998 Jun;51(3):155-9. doi: 10.1136/mp.51.3.155.
AIMS
Human herpes virus 8 (HHV-8) is the infectious agent implicated in the pathogenesis of Kaposi's sarcoma, although its mode of action is unclear. Recent work indicates that the HHV-8 genome encodes a viral Bcl-2 homologue (v-Bcl-2). The aim of this study was to explore Bcl-2 expression in Kaposi's sarcoma using a unique set of HHV-8 positive and negative cases, and to determine whether there is a relation with p53 expression.
METHODS
Up to 18 specimens from 17 patients were selected. HHV-8 status was determined using nested polymerase chain reaction (PCR) to the open reading frame (ORF) 26, with further confirmation by TaqMan PCR. In addition, Bcl-2 and p53 immunohistochemistry were performed using standard protocols.
RESULTS
The results suggest that Bcl-2 and p53 expression is independent of HHV-8 status. In addition, there does not appear to be a direct correlation with disease stage.
CONCLUSIONS
HHV8 histopathogenesis is likely to be a multifactorial complex process, which may be mediated in part by viral genes and apoptosis regulating homologues.
目的
人类疱疹病毒8型（HHV - 8）是与卡波西肉瘤发病机制相关的感染因子，但其作用方式尚不清楚。最近的研究表明，HHV - 8基因组编码一种病毒Bcl - 2同源物（v - Bcl - 2）。本研究的目的是利用一组独特的HHV - 8阳性和阴性病例来探讨卡波西肉瘤中Bcl - 2的表达情况，并确定其与p53表达是否存在关联。
方法
从17例患者中选取了多达18个标本。采用巢式聚合酶链反应（PCR）检测开放阅读框（ORF）26来确定HHV - 8状态，并通过TaqMan PCR进一步确认。此外，使用标准方案进行Bcl - 2和p53免疫组织化学检测。
结果
结果表明，Bcl - 2和p53的表达与HHV - 8状态无关。此外，似乎与疾病分期也没有直接相关性。
结论
HHV8的组织病理学发病机制可能是一个多因素的复杂过程，可能部分由病毒基因和凋亡调节同源物介导。
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