Shabalkin I P
Arkh Anat Gistol Embriol. 1976 Sep;71(9):29-35.
Method of autoradiography was used in order to study the kinetics of population of the cornea epithelium cells of mice. Intervals of different duration were found to exist in the DNA synthesis within the limits of S-period of one mitotic cycle. On the basis of personal and literature data a hypothesis has been put forward of a successive pattern of replication in the cells of eukaryots according to which synthesis of a fragment of the DNA daughter thread (or a chromosome subunit) occurs at each moment in a restricted site of a single matrix thread of DNA (matrix chromosome subunit). No DNA synthesis takes place at this moment in the complementary site of the second matrix thread (matrix chromosome subunit), the fragment (chromosome subunit) of one matrix thread being somewhat larger than the complementary fragment (chromosome subunit of the other matrix thread.
为了研究小鼠角膜上皮细胞群体的动力学，采用了放射自显影法。发现在一个有丝分裂周期的S期范围内，DNA合成存在不同持续时间的间隔。根据个人和文献资料，提出了真核细胞中连续复制模式的假说，根据该假说，DNA子链（或染色体亚基）片段的合成在每个时刻发生在单个DNA基质链（基质染色体亚基）的受限位点。此时在第二条基质链（基质染色体亚基）的互补位点不发生DNA合成，一条基质链的片段（染色体亚基）比另一条基质链的互补片段（染色体亚基）稍大。
