有证据表明白喉毒素产生的调控作用发生在转录水平。
Evidence that the regulation of diphtheria toxin production is directed at the level of transcription.
作者信息
Murphy J R, Michel J L, Teng M
出版信息
J Bacteriol. 1978 Aug;135(2):511-6. doi: 10.1128/jb.135.2.511-516.1978.
Abstract
It has been known for several decades that iron inhibits the production of diphtheria toxin by Corynebacterium diphtheriae by preventing expression at maximal levels. We examined the inhibition kinetics of toxin production after the addition of either iron or rifampin to iron-limited cultures of C7 (betatox+). Iron-mediated inhibition of toxin production was found to be linear within the range of 16 nM to 16 micron. The inhibition kinetics following the addition of iron or rifampin was almost identical. [3H]RNA extracted from iron-limited toxigenic C. diphtheriae was found to hybridize to a greater extent to corynephage beta DNA than either [3H]RNA extracted from toxigenic C. diphtheriae before the onset of toxin production or [3H]RNA extracted from nonlysogenic, nontoxigenic C. diphtheriae.
摘要
几十年来人们一直知道，铁通过阻止白喉棒状杆菌达到最大表达水平来抑制其产生白喉毒素。我们在铁限制的C7（β毒素阳性）培养物中添加铁或利福平后，研究了毒素产生的抑制动力学。发现铁介导的毒素产生抑制在16 nM至16微米范围内呈线性。添加铁或利福平后的抑制动力学几乎相同。从铁限制的产毒白喉棒状杆菌中提取的[3H]RNA，与从毒素产生开始前的产毒白喉棒状杆菌中提取的[3H]RNA或从非溶原性、非产毒白喉棒状杆菌中提取的[3H]RNA相比，与棒状噬菌体β DNA的杂交程度更高。
相似文献
1
Evidence that the regulation of diphtheria toxin production is directed at the level of transcription.有证据表明白喉毒素产生的调控作用发生在转录水平。
J Bacteriol. 1978 Aug;135(2):511-6. doi: 10.1128/jb.135.2.511-516.1978.
2
Isolation from corynebacterium diphtheriae C7(beta) of bacterial mutants that produce toxin in medium with excess iron.从白喉棒状杆菌C7（β）中分离出在含铁过量的培养基中产生毒素的细菌突变体。
Infect Immun. 1977 Oct;18(1):203-9. doi: 10.1128/iai.18.1.203-209.1977.
3
Molecular characterization of diphtheria toxin repressor (dtxR) genes present in nontoxigenic Corynebacterium diphtheriae strains isolated in the United Kingdom.对在英国分离出的非产毒白喉棒状杆菌菌株中存在的白喉毒素阻遏物（dtxR）基因进行分子特征分析。
J Clin Microbiol. 2005 Jan;43(1):223-8. doi: 10.1128/JCM.43.1.223-228.2005.
4
Coordinate regulation of siderophore and diphtheria toxin production by iron in Corynebacterium diphtheriae.铁对白喉棒状杆菌中铁载体和白喉毒素产生的协同调节作用
Microb Pathog. 1990 Oct;9(4):267-73. doi: 10.1016/0882-4010(90)90015-i.
5
Iron-dependent regulation of diphtheria toxin and siderophore expression by the cloned Corynebacterium diphtheriae repressor gene dtxR in C. diphtheriae C7 strains.克隆的白喉棒状杆菌阻遏基因dtxR对白喉毒素和铁载体表达的铁依赖性调控作用，在白喉棒状杆菌C7菌株中。
Infect Immun. 1991 Jun;59(6):1899-904. doi: 10.1128/iai.59.6.1899-1904.1991.
6
Regulation of toxinogenesis in Corynebacterium diphtheriae: mutations in the bacterial genome that alter the effects of iron on toxin production.白喉棒状杆菌中毒素生成的调控：细菌基因组中的突变改变了铁对毒素产生的影响。
J Bacteriol. 1983 Apr;154(1):245-52. doi: 10.1128/jb.154.1.245-252.1983.
7
Corynebacterium ulcerans and Corynebacterium pseudotuberculosis responses to DNA probes derived from corynephage beta and Corynebacterium diphtheriae.溃疡棒状杆菌和伪结核棒状杆菌对源自β棒状噬菌体和白喉棒状杆菌的DNA探针的反应。
Infect Immun. 1984 Aug;45(2):511-7. doi: 10.1128/iai.45.2.511-517.1984.
8
Regulation of toxinogenesis in Corynebacterium diphtheriae. I. Mutations in bacteriophage beta that alter the effects of iron on toxin production.白喉棒状杆菌毒素生成的调控。I. 噬菌体β中的突变改变铁对毒素产生的影响。
J Virol. 1981 Mar;37(3):936-45. doi: 10.1128/JVI.37.3.936-945.1981.
9
Isolation and partial characterization of a corynebacteriophage beta, tox operator constitutive-like mutant lysogen of Corynebacterium diphtheriae.白喉棒状杆菌的一种棒状噬菌体β、毒力操纵子组成型样突变溶原菌的分离与部分特性分析
J Virol. 1976 Apr;18(1):235-44. doi: 10.1128/JVI.18.1.235-244.1976.
10
[Use of a DNA-DNA hybridization method for studying Corynebacterium diphtheriae strains].[使用DNA-DNA杂交方法研究白喉棒状杆菌菌株]
Mol Gen Mikrobiol Virusol. 1994 Nov-Dec(6):27-9.
引用本文的文献
1
Effect of iron on cytolysin A expression in Salmonella enterica serovar Typhi.铁对伤寒沙门氏菌中细胞溶素A表达的影响。
J Microbiol. 2009 Aug;47(4):479-85. doi: 10.1007/s12275-009-0039-4. Epub 2009 Sep 9.
2
Regulation and activity of a zinc uptake regulator, Zur, in Corynebacterium diphtheriae.白喉棒状杆菌中锌摄取调节蛋白Zur的调控与活性
J Bacteriol. 2009 Mar;191(5):1595-603. doi: 10.1128/JB.01392-08. Epub 2008 Dec 12.
3
Characterization of mutations that inactivate the diphtheria toxin repressor gene (dtxR).使白喉毒素阻遏基因（dtxR）失活的突变的特征分析。
Infect Immun. 1994 May;62(5):1600-8. doi: 10.1128/iai.62.5.1600-1608.1994.
4
Cloning, sequence, and footprint analysis of two promoter/operators from Corynebacterium diphtheriae that are regulated by the diphtheria toxin repressor (DtxR) and iron.对白喉棒状杆菌中受白喉毒素阻遏物（DtxR）和铁调控的两个启动子/操纵子的克隆、测序及足迹分析
J Bacteriol. 1994 Feb;176(4):1141-9. doi: 10.1128/jb.176.4.1141-1149.1994.
5
High-yield production of diphtheria toxin mutants by high-density culture of C7 (beta)tox+ strains grown in a non-deferrated medium.通过在非去铁培养基中高密度培养C7（β）毒素+菌株高产白喉毒素突变体。
Appl Microbiol Biotechnol. 1995 Apr;43(1):83-8. doi: 10.1007/BF00170627.
6
Use of cir-lac operon fusions to study transcriptional regulation of the colicin Ia receptor in Escherichia coli K-12.利用cir-lac操纵子融合来研究大肠杆菌K-12中大肠菌素Ia受体的转录调控。
J Bacteriol. 1981 Jan;145(1):647-50. doi: 10.1128/jb.145.1.647-650.1981.
7
Cotranslational secretion of diphtheria toxin and alkaline phosphatase in vitro: involvement of membrane protein(s).体外白喉毒素与碱性磷酸酶的共翻译分泌：膜蛋白的参与
J Bacteriol. 1980 Mar;141(3):1142-7. doi: 10.1128/jb.141.3.1142-1147.1980.
8
Regulation of toxinogenesis in Corynebacterium diphtheriae. II. Genetic mapping of a tox regulatory mutation in bacteriophage beta.白喉棒状杆菌毒素生成的调控。II. β噬菌体中毒素调控突变的基因定位。
J Virol. 1981 Mar;37(3):946-54. doi: 10.1128/JVI.37.3.946-954.1981.
9
Regulation of toxinogenesis in Corynebacterium diphtheriae. I. Mutations in bacteriophage beta that alter the effects of iron on toxin production.白喉棒状杆菌毒素生成的调控。I. 噬菌体β中的突变改变铁对毒素产生的影响。
J Virol. 1981 Mar;37(3):936-45. doi: 10.1128/JVI.37.3.936-945.1981.
10
Regulation of toxinogenesis in Corynebacterium diphtheriae: mutations in the bacterial genome that alter the effects of iron on toxin production.白喉棒状杆菌中毒素生成的调控：细菌基因组中的突变改变了铁对毒素产生的影响。
J Bacteriol. 1983 Apr;154(1):245-52. doi: 10.1128/jb.154.1.245-252.1983.
本文引用的文献
1
[Growth and the genesis of toxins].[生长与毒素的产生]
Ann Inst Pasteur (Paris). 1954 Dec;87(6):599-616.
2
Mutation in the structural gene for diphtheria toxin carried by temperate phage .温和噬菌体携带的白喉毒素结构基因中的突变。
Nat New Biol. 1971 Sep 1;233(35):8-11. doi: 10.1038/newbio233008a0.
3
Transcription of the tryptophan operon in Escherichia coli: rifampicin as an inhibitor of initiation.大肠杆菌中色氨酸操纵子的转录：利福平作为起始抑制剂
J Mol Biol. 1970 Mar;48(3):525-31. doi: 10.1016/0022-2836(70)90064-1.
4
Genetic analysis of tox+ and tox- bacteriophages of Corynebacterium diphtheriae.白喉棒状杆菌产毒和无毒噬菌体的遗传分析。
J Virol. 1969 Jun;3(6):586-98. doi: 10.1128/JVI.3.6.586-598.1969.
5
System for the investigation of the bacteriophage-directed synthesis of diphtherial toxin.用于研究噬菌体指导的白喉毒素合成的系统。
J Bacteriol. 1967 Feb;93(2):722-30. doi: 10.1128/jb.93.2.722-730.1967.
6
Phage-directed synthesis of diphtherial toxin in non-toxinogenic Corynebacterium diphtheriae.噬菌体指导非产毒素白喉棒状杆菌合成白喉毒素。
Nature. 1966 May 28;210(5039):911-3. doi: 10.1038/210911a0.
7
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.一种用于检测固定在膜上的DNA与RNA杂交体的定量分析方法。
J Mol Biol. 1965 Jul;12(3):829-42. doi: 10.1016/s0022-2836(65)80331-x.
8
Iron transport in Escherichia coli and its relation to the repression of 2,3-dihydroxy-N-benzoyl-L-serine synthetase.大肠杆菌中的铁转运及其与2,3 - 二羟基 - N - 苯甲酰 - L - 丝氨酸合成酶抑制的关系。
Arch Biochem Biophys. 1971 Feb;142(2):399-406. doi: 10.1016/0003-9861(71)90503-0.
9
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.聚乙二醇存在下噬菌体的快速沉降及其在大规模病毒纯化中的应用
Virology. 1970 Mar;40(3):734-44. doi: 10.1016/0042-6822(70)90218-7.
10
Interaction of rifamycin with bacterial RNA polymerase.利福霉素与细菌RNA聚合酶的相互作用。
Proc Natl Acad Sci U S A. 1968 Oct;61(2):667-73. doi: 10.1073/pnas.61.2.667.
Zotero 插件