Groman N, Schiller J, Russell J
Infect Immun. 1984 Aug;45(2):511-7. doi: 10.1128/iai.45.2.511-517.1984.
Strains of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis (Corynebacterium ovis) were examined for the production of diphtheria toxin. A majority of C. ulcerans strains (25 of 37) and 1 C. pseudotuberculosis strain (1 of 14) gave a positive Elek test for diphtheria toxin, and for all strains but 1, production of diphtheria toxin was inhibited at the same level of Fe2+ as was the Corynebacterium diphtheriae control. All Elek-positive cultures as well as two Elek-negative isolates of C. ulcerans gave a positive signal when hybridized with a DNA probe unambiguous for the diphtheria toxin gene (tox) under conditions of high stringency. The majority of probe-positive C. ulcerans strains contained three or more DNA restriction fragments that hybridized with converting corynephage beta, suggesting that in C. ulcerans as in C. diphtheriae there may be a relationship between toxinogeny and carriage of beta-related phage. Selected strains of C. diphtheriae, C. ulcerans, and C. pseudotuberculosis were examined for DNA homology by a semiquantitative technique. There was very little homology between C. diphtheriae and members of the other two species. Strains of C. ulcerans and C. pseudotuberculosis, although more closely related, appeared to belong to distinct species as well.
对溃疡棒状杆菌和假结核棒状杆菌（羊棒状杆菌）菌株进行了白喉毒素产生情况的检测。大多数溃疡棒状杆菌菌株（37株中的25株）和1株假结核棒状杆菌菌株（14株中的1株）对白喉毒素的Elek试验呈阳性，除1株外的所有菌株，其白喉毒素的产生在与白喉棒状杆菌对照相同水平的Fe2+下受到抑制。当在高严格条件下与对白喉毒素基因（tox）明确无误的DNA探针杂交时，所有Elek阳性培养物以及两株溃疡棒状杆菌的Elek阴性分离株均给出阳性信号。大多数探针阳性的溃疡棒状杆菌菌株含有三个或更多与转化性β棒状噬菌体杂交的DNA限制性片段，这表明在溃疡棒状杆菌中，如同在白喉棒状杆菌中一样，产毒素能力与β相关噬菌体的携带之间可能存在关联。通过半定量技术对选定的白喉棒状杆菌、溃疡棒状杆菌和假结核棒状杆菌菌株进行了DNA同源性检测。白喉棒状杆菌与其他两个物种的成员之间几乎没有同源性。溃疡棒状杆菌和假结核棒状杆菌菌株虽然关系更密切，但似乎也属于不同的物种。
