Mayfield M P, Shah T, Flannigan G M, Hamilton Stewart P A, Bibby M C
Clinical Oncology Unit, University of Bradford, Bradford, BD7 1DP, UK.
Int J Mol Med. 1998 May;1(5):835-40. doi: 10.3892/ijmm.1.5.835.
This study investigated whether the enzyme telomerase is active in bladder tumours, whether there is any correlation between activity and grade, and whether the enzyme is expressed in non-malignant conditions. Fifty-two patients undergoing cystoscopy or TURBT at a district general hospital were included, 25 with current bladder tumours, 13 with previous but no current tumours, and 14 with non-malignant pathology. Specimens were analysed by the telomerase repeat amplification protocol (TRAP assay), a highly-sensitive polymerase chain reaction (PCR)-based assay, and a commercially-available ELISA kit. Telomerase activity was detected in 80% of bladder tumours, more frequently in moderate- or poorly-differentiated (93%) than well-differentiated (56%) tumours. Activity was not uniform across individual tumours. Telomerase was also frequently (61%) detected in inflammatory lesions found in patients being followed up for previous bladder tumours, and in two (14%) patients with benign pathologies. In conclusion, telomerase was frequently but not uniformly detected in bladder tumours; its presence was not specific to malignancy. There is a possible correlation between tumour grade and telomerase activity.
本研究调查了端粒酶在膀胱肿瘤中是否具有活性，活性与肿瘤分级之间是否存在关联，以及该酶在非恶性病变中是否表达。纳入了一家地区综合医院接受膀胱镜检查或经尿道膀胱肿瘤电切术（TURBT）的52例患者，其中25例患有当前膀胱肿瘤，13例曾患膀胱肿瘤但目前无肿瘤，14例有非恶性病变。通过端粒酶重复序列扩增法（TRAP检测）、一种基于聚合酶链反应（PCR）的高灵敏度检测方法以及一种市售酶联免疫吸附测定（ELISA）试剂盒对标本进行分析。在80%的膀胱肿瘤中检测到端粒酶活性，在中分化或低分化肿瘤（93%）中比高分化肿瘤（56%）中更常见。个体肿瘤中的活性并不一致。在因既往膀胱肿瘤接受随访的患者中发现的炎性病变以及两名（14%）患有良性病变的患者中也经常（61%）检测到端粒酶。总之，在膀胱肿瘤中经常检测到端粒酶，但并不一致；其存在并非恶性肿瘤所特有。肿瘤分级与端粒酶活性之间可能存在关联。
