Holme S, Elfath M D, Whitley P
American Red Cross, Mid-Atlantic Region, Norfolk, VA, USA.
Vox Sang. 1998;75(3):212-7.
New technological developments make it possible to collect red blood cells (RBCs) by apheresis, which allows for better product consistency and has the potential for improved RBC quality. The purpose of these studies was to evaluate the quality and consistency of units of RBCs collected by apheresis using the MCS+(R) machine (Haemonetics Corp., Braintree, Mass., USA).
Two studies were performed. In study 1 (n = 10), using containers and CP2D/AS-3 solutions from Medsep Corp. (Covina, Calif. USA), one-unit apheresis RBCs were compared to manually collected RBCs in a random crossover design. In study 2 (n = 12), 6 subjects had one unit collected, while the remaining 6 subjects had two units of RBCs collected with comparison to previously manually collected RBCs from the same donors. Haemonetics containers and solutions were used in study 2.
Low RBC volume variability was found for the apheresis collections with a standard deviation of only 6 ml difference between actual and target volumes. Combining the data from the two studies (n = 21 pairs), at 42 days of storage, the apheresis units showed slightly lower hemolysis (0.44+/-0.26 vs. 0.61+/-0.50%), lower supernatant potassium levels (50+/-3 vs. 53+/-3 mEq/l), and improved tolerance to osmotic shock (47+/-3 vs. 49+/-3%) as compared to manual units (p < 0.05). There was no statistically significant difference in RBC ATP (3.0+/-0.6 vs. 2.9+/-0.5 micromol/g Hb) or in 24-hour percent recoveries (81+/-6 for apheresis vs. 81+/-4% for apheresis red cells). Apheresis RBC quality was not affected by the manufacturer (Haemonetics vs. Medsep) of solutions and containers.
RBC units collected by apheresis demonstrated low variability in volume of RBC mass collected, and showed similar RBC properties as compared to manually collected RBCs after processing and after 42 days of storage.
新技术的发展使得通过血液成分单采术采集红细胞（RBC）成为可能，这能实现更好的产品一致性，并有可能提高红细胞质量。这些研究的目的是评估使用MCS+(R)机器（美国马萨诸塞州布伦特里市的Haemonetics公司）通过血液成分单采术采集的红细胞单位的质量和一致性。
进行了两项研究。在研究1（n = 10）中，使用美国加利福尼亚州科维纳市Medsep公司的容器和CP2D/AS-3溶液，采用随机交叉设计，将单采红细胞与手工采集的红细胞进行比较。在研究2（n = 12）中，6名受试者采集1个单位，其余6名受试者采集2个单位的红细胞，并与同一供体先前手工采集的红细胞进行比较。研究2中使用了Haemonetics公司的容器和溶液。
血液成分单采术采集的红细胞体积变异性较低，实际体积与目标体积之间的标准差仅相差6 ml。综合两项研究的数据（n = 21对），在储存42天时，与手工采集的红细胞相比，单采红细胞单位的溶血率略低（0.44±0.26%对0.61±0.50%），上清钾水平较低（50±3对53±3 mEq/l），对渗透压休克的耐受性有所改善（47±3%对49±3%）（p < 0.05）。红细胞ATP（3.0±0.6对2.9±0.5 μmol/g Hb）或24小时回收率（单采术为81±6%，单采红细胞为81±4%）无统计学显著差异。单采红细胞质量不受溶液和容器制造商（Haemonetics公司与Medsep公司）的影响。
通过血液成分单采术采集的红细胞单位在采集的红细胞量体积方面变异性较低，在处理后和储存42天后，与手工采集的红细胞相比，显示出相似的红细胞特性。
