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Cell yield and superoxide dismutase activity of the marine yeast Debaryomyces hansenii under different culture conditions.

作者信息

Hernández-Saavedra NY, Ochoa JL

机构信息

Centro de Investigaciones Biológicas del Noroeste, División de Biologia Experimental, Unidad de Patologia Marina, A.P. 128, La Paz 23000, Baja California Sur, México

出版信息

J Mar Biotechnol. 1998 Dec;6(4):255-259.

PMID:9852622
Abstract

The effect of aeration, pH, stirring rate, and temperature on the biomass production and superoxide dismutase (SOD) activity of the marine yeast Debaryomyces hansenii strain C-11 was determined. The cell biomass yield was approximately 50% in a seawater-formulated medium using glucose as the carbon source. The SOD activity increased by application of a pulse of oxygen or 0.8 mM sulfate copper into the chemical reactor. The SOD enzyme had an activity of 400 units/mg of protein in a crude extract produced under such conditions, the best activity ever reported for this enzyme in a crude preparation.

摘要

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