Immunology & Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, 23096, La Paz, BCS, Mexico.
Laboratorio de Sanidad Animal, Universidad Autónoma de Baja California Sur, Carretera al Sur km. 5.5, Col. Mezquitito, 23080, La Paz, BCS, Mexico.
Appl Microbiol Biotechnol. 2019 Mar;103(5):2339-2352. doi: 10.1007/s00253-019-09621-5. Epub 2019 Jan 18.
Several marine Debaryomyces hansenii strains have shown probiotic effects on aquatic animals, and D. hansenii-derived β-glucans have recently provided immunostimulant effects on goat leukocytes. This study assessed the probiotic effects of live yeast D. hansenii CBS 8339 on newborn goats administered orally, and subsequently challenged in vitro with Escherichia coli. D. hansenii CBS 8339 demonstrated the capacity to survive gastrointestinal tract conditions (bile salts and acid pH tolerance) and adhere to goat intestine. Twelve Saanen × Nubian crossbred newborn goats (2.9 ± 0.47 kg) were fed with a controlled diet or D. hansenii (0.7 g/kg body weight per day)-supplemented milk for 30 days. Blood samples of newborn goats were taken at days 15 and 30, and peripheral blood leukocytes were isolated for bacterial challenge, and immunological and antioxidant analyses. Despite cell viability was higher in leukocytes of goat kids fed with the yeast supplement, protection against E. coli challenge was not significantly affected. On the other hand, at day 15, oral administration of D. hansenii enhanced respiratory burst and catalase activity and increased superoxide dismutase activity after challenge. In contrast, at day 30, administration of the yeast supplement increased peroxidase activity and enhanced nitric oxide production and catalase activity after challenge. Finally, the yeast-supplemented diet upregulated the expression of the receptor genes TLR (2, 4, 6), modulator genes Raf.1, Syk, and Myd88, transcription factor gene AP-1, and cytokine genes IL-1β and TNF-α only at day 15 in leukocytes from unchallenged goat kids. These results demonstrated that a short time (15 days) of orally administering the probiotic D. hansenii CBS 8339 to newborn goats stimulated innate immune and antioxidant parameters and the expression of immune-related gene signaling pathways.
几种海洋德巴利酵母汉逊氏酵母菌株对水生动物表现出益生菌作用，而源自德巴利酵母汉逊氏酵母的β-葡聚糖最近对山羊白细胞提供了免疫刺激作用。本研究评估了活酵母德巴利酵母汉逊氏酵母 CBS 8339 对口服给予的新生山羊的益生菌作用，随后在体外用大肠杆菌进行挑战。德巴利酵母汉逊氏酵母 CBS 8339 表现出在胃肠道条件（胆汁盐和酸 pH 值耐受）下生存和黏附于山羊肠道的能力。12 只萨能 ×努比亚杂交新生山羊（2.9 ± 0.47 kg）用控制饮食或添加德巴利酵母汉逊氏酵母（0.7 g/kg 体重/天）的牛奶喂养 30 天。在第 15 天和第 30 天采集新生山羊的血液样本，并分离外周血白细胞进行细菌挑战和免疫及抗氧化分析。尽管添加酵母补充剂的山羊白细胞的细胞活力更高，但对大肠杆菌挑战的保护作用没有显著影响。另一方面，在第 15 天，口服德巴利酵母汉逊氏酵母增强了呼吸爆发和过氧化氢酶活性，并在挑战后增加了超氧化物歧化酶活性。相比之下，在第 30 天，添加酵母补充剂增加了过氧化物酶活性，并在挑战后增强了一氧化氮产生和过氧化氢酶活性。最后，酵母补充饮食仅在第 15 天上调了未受挑战的山羊白细胞中 TLR（2、4、6）受体基因、Raf.1、Syk 和 Myd88 调节剂基因、AP-1 转录因子基因和 IL-1β 和 TNF-α 细胞因子基因的表达。这些结果表明，在新生山羊中口服给予益生菌德巴利酵母汉逊氏酵母 CBS 8339 短时间（15 天）刺激了先天免疫和抗氧化参数以及免疫相关基因信号通路的表达。
