Giri A, Khynriam D, Prasad S B
Department of Zoology, School of Life Sciences, North-Eastern Hill University, Shillong, India.
Mutat Res. 1998 Nov 3;421(2):139-48. doi: 10.1016/s0027-5107(98)00158-4.
In present studies the development of chromosomal aberrations, micronuclei in bone marrow cells and sperm head abnormalities were used as mutagenic bioassay in Swiss albino mice treated with cisplatin alone or ascorbic plus cisplatin. It was noted that in the combined treated hosts the frequency of all the mutagenic parameters were always significantly less than that treated with cisplatin alone. These findings suggest a protective role of ascorbic acid against cisplatin induced mutagenic potentials. Interestingly, in combined treated hosts glutathione (GSH) level in bone marrow cells increased significantly which may suggest a possible mechanism of ascorbic acid mediated protection against cisplatin induced mutagenic potentials in the hosts.
在目前的研究中，染色体畸变、骨髓细胞中的微核以及精子头部异常的发生情况被用作瑞士白化小鼠的致突变生物测定指标，这些小鼠单独接受顺铂治疗，或同时接受抗坏血酸加顺铂治疗。值得注意的是，在联合治疗的宿主中，所有致突变参数的频率总是显著低于单独使用顺铂治疗的宿主。这些发现表明抗坏血酸对顺铂诱导的致突变潜能具有保护作用。有趣的是，在联合治疗的宿主中，骨髓细胞中的谷胱甘肽（GSH）水平显著升高，这可能表明抗坏血酸介导的对宿主顺铂诱导的致突变潜能的保护作用的一种可能机制。
