Ergün E L, Ercan M T, Aşansü A, Unsal I S
Department of Nuclear Medicine, Hacettepe University Medical Faculty, Ankara, Turkey.
Nucl Med Commun. 1998 Jul;19(7):665-70.
In this pilot study, 99Tcm-labelled human immunoglobulin G (99Tcm-HIG) was evaluated as a lymphoscintigraphic agent in five rabbits. It was injected intradermally into the web space of the hind legs of the rabbits (37 MBq/0.1 ml). Sequential scintigrams were obtained using a gamma camera for 120 min. The injection site and the hind legs were massaged post-injection. Blood samples were obtained at 5, 15, 30, 60, 90 and 120 min. Two of the rabbits were killed after 2 h. Their organs were weighed and tissue specimens were obtained, weighed and counted against a standard using a gamma counter. The lymph channels and the lymph nodes were well visualized on the scintigrams. The background activity was very low, making interpretation easier. About 30% of the injected dose migrated from the injection site by 2 h. The mean popliteal lymph node uptake was 5.71 +/- 4.62% per gram of tissue. The lymph node to other tissue concentration ratios were very high, ranging from 63:1 for the kidneys to 1099:1 for the heart. We conclude that 99Tcm-HIG is a promising new agent for the visualization of the lymphatic system due to its easy labelling procedure, the stability of the label, its widespread availability and good image quality. It may potentially be useful in detecting and evaluating inflammatory lymph nodes.
在这项初步研究中，对99锝标记的人免疫球蛋白G（99Tcm-HIG）作为淋巴闪烁造影剂在5只兔子身上进行了评估。将其皮内注射到兔子后腿的蹼间隙（37 MBq/0.1 ml）。使用γ相机连续采集120分钟的闪烁图。注射后对注射部位和后腿进行按摩。在5、15、30、60、90和120分钟采集血样。2小时后处死2只兔子。称取它们的器官重量，并获取组织标本，称重后使用γ计数器与标准品进行计数。在闪烁图上，淋巴管和淋巴结清晰可见。本底活性非常低，便于解读。到2小时时，约30%的注射剂量从注射部位迁移。腘窝淋巴结的平均摄取量为每克组织5.71±4.62%。淋巴结与其他组织的浓度比非常高，肾脏为63:1，心脏为1099:1。我们得出结论，99Tcm-HIG因其标记程序简便、标记稳定性好、广泛可得且图像质量良好，是一种用于淋巴系统显像的有前景的新型试剂。它可能在检测和评估炎性淋巴结方面具有潜在用途。
