编码大肠杆菌磷脂酰甘油磷酸合酶的野生型和突变等位基因的一级结构。
Primary structures of the wild-type and mutant alleles encoding the phosphatidylglycerophosphate synthase of Escherichia coli.
作者信息
Usui M, Sembongi H, Matsuzaki H, Matsumoto K, Shibuya I
机构信息
Department of Biochemistry, Saitama University, Urawa, Japan.
出版信息
J Bacteriol. 1994 Jun;176(11):3389-92. doi: 10.1128/jb.176.11.3389-3392.1994.
Abstract
The nucleotide sequence of the Escherichia coli pgsA gene, encoding phosphatidylglycerophosphate synthase, is revised to code for an enzyme of 182 amino acid residues, instead of the 216 of a previous work (A. S. Gopalakrishnan, Y.-C. Chen, M. Temkin, and W. Dowhan, J. Biol. Chem. 261:1329-1338, 1986). The revised structure now explains the properties of the enzyme. Three pgsA mutants of different phenotypes were also analyzed: pgsA3, pgsA36, and pgsA10 have single-base replacements in codons 60 (Thr-->Pro), 1 (ATG-->ATA), and 92 (Thr-->Ile), respectively.
摘要
编码磷脂酰甘油磷酸合酶的大肠杆菌pgsA基因的核苷酸序列经修订后编码一种由182个氨基酸残基组成的酶，而非先前研究（A. S. 戈帕拉克里什南、Y.-C. 陈、M. 坦金和W. 多曼，《生物化学杂志》261:1329 - 1338, 1986）中所报道的216个氨基酸残基。修订后的结构如今解释了该酶的特性。还对三个不同表型的pgsA突变体进行了分析：pgsA3、pgsA36和pgsA10分别在密码子60（苏氨酸→脯氨酸）、1（ATG→ATA）和92（苏氨酸→异亮氨酸）处发生了单碱基替换。
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Primary structures of the wild-type and mutant alleles encoding the phosphatidylglycerophosphate synthase of Escherichia coli.编码大肠杆菌磷脂酰甘油磷酸合酶的野生型和突变等位基因的一级结构。
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本文引用的文献
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