Shi S R, Cote R J, Taylor C R
Department of Pathology, University of Southern California School of Medicine, Los Angeles, 90033, USA.
Auris Nasus Larynx. 1998 Dec;25(4):425-43. doi: 10.1016/s0385-8146(98)00042-x.
The use of immunohistochemistry (IHC) in routinely processed celloidin-embedded human temporal bone section has created a fruitful field in understanding the pathogenesis and pathophysiology of the human inner ear at a molecular level since the early 1990s when the antigen retrieval (AR) technique was developed. This review article focuses on several critical technical issues of AR technique based predominantly on our experiences and suggestions concerning further development and standardization of AR-IHC for IHC study of human temporal bone section, as well as other tissues embedded in celloidin. Examples of using AR-IHC in human temporal bone sections collected include our unpublished data in order to indicate the potential utility of this novel method. Suggestions of further development of AR techniques are proposed for references and comments.
自20世纪90年代初抗原修复（AR）技术发展以来，免疫组织化学（IHC）在常规处理的火棉胶包埋人颞骨切片中的应用，在分子水平上为理解人类内耳的发病机制和病理生理学开辟了一个富有成果的领域。这篇综述文章主要基于我们的经验，重点探讨了AR技术的几个关键技术问题，并就用于人颞骨切片及其他火棉胶包埋组织的免疫组化研究的AR免疫组化的进一步发展和标准化提出了建议。收集的人颞骨切片中使用AR免疫组化的实例包括我们未发表的数据，以表明这种新方法的潜在用途。对AR技术的进一步发展提出了建议，以供参考和评论。
