Morris J M, Gopaul N K, Endresen M J, Knight M, Linton E A, Dhir S, Anggård E E, Redman C W
Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, Oxford, UK.
Br J Obstet Gynaecol. 1998 Nov;105(11):1195-9. doi: 10.1111/j.1471-0528.1998.tb09974.x.
To determine whether circulating markers of oxidative stress are elevated in pre-eclampsia when appropriate precautions are taken to prevent in vitro oxidation
A prospective study.
Nuffield Department of Obstetrics and Gynaecology, Oxford and The William Harvey Institute, London.
Three groups of women: those with pre-eclampsia (n = 19), control pregnant women (n = 19) matched for gestation, age and parity and a group of non pregnant individuals of reproductive age (n = 7).
Citrated plasma was stored at -80 degrees C with 20 micromol beta hydroxytoluene to prevent auto-oxidation. Plasma samples were assayed for levels of 8 epi-prostaglandin F2alpha, lipid hydroperoxides, malondialdehyde and also the lipid soluble antioxidant vitamin E.
There were no differences in 8 epi-prostaglandin F2alpha, lipid peroxide or malondialdehyde levels between the groups of women with pre-eclampsia and those acting as pregnant controls. However, lipid hydroperoxides and malondialdehyde were significantly raised in both pre-eclampsia and normal pregnancy, compared with nonpregnant women. Vitamin E levels were similar in women with pre-eclampsia and those with a normal pregnancy, but in both groups levels were significantly higher than in nonpregnant women.
Circulating markers of oxidative stress are raised in normal pregnancy and pre-eclampsia.
在采取适当预防措施以防止体外氧化的情况下，确定子痫前期患者循环氧化应激标志物是否升高
前瞻性研究。
牛津大学纳菲尔德妇产科系和伦敦威廉·哈维研究所。
三组女性：子痫前期患者（n = 19）、孕周、年龄和产次相匹配的对照孕妇（n = 19）以及一组育龄非孕个体（n = 7）。
将枸橼酸盐血浆与20微摩尔β - 羟基甲苯一起储存在 - 80℃以防止自动氧化。检测血浆样本中8 - 表前列腺素F2α、脂质氢过氧化物、丙二醛的水平以及脂溶性抗氧化剂维生素E的水平。
子痫前期患者组与作为对照的孕妇组之间，8 - 表前列腺素F2α、脂质过氧化物或丙二醛水平没有差异。然而，与非孕女性相比，子痫前期患者和正常孕妇的脂质氢过氧化物和丙二醛水平均显著升高。子痫前期患者和正常孕妇的维生素E水平相似，但两组的水平均显著高于非孕女性。
正常妊娠和子痫前期患者的循环氧化应激标志物均升高。
