两种用于检测溃疡分枝杆菌的聚合酶链反应的比较。
Comparison of two PCRs for detection of Mycobacterium ulcerans.
作者信息
Guimaraes-Peres A, Portaels F, de Rijk P, Fissette K, Pattyn S R, van Vooren J, Fonteyne P
机构信息
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.
出版信息
J Clin Microbiol. 1999 Jan;37(1):206-8. doi: 10.1128/JCM.37.1.206-208.1999.
Abstract
Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.
摘要
通过使用65份临床标本的集合，对两种用于检测溃疡分枝杆菌的巢式PCR进行了比较。第一种方法扩增编码16S rRNA的基因，第二种方法扩增重复DNA序列。细菌学检查、培养、16S rRNA基因PCR和重复序列PCR的敏感性分别为29%、34%、80%和85%。与16S rRNA基因PCR相比，重复序列PCR更快、更易于操作且成本更低。
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