Lovgren M, Dell'Acqua L, Palacio R, Echániz-Aviles G, Soto-Noguerón A, Castañeda E, Agudelo C I, Heitmann I, Brandileone M C, Zanella R C, Rossi A, Pace J, Talbot J A
National Centre for Streptococcus, Edmonton, Canada.
J Clin Microbiol. 1999 Jan;37(1):215-7. doi: 10.1128/JCM.37.1.215-217.1999.
An international, multicenter study compared trimethoprim-sulfamethoxazole MICs for 743 Streptococcus pneumoniae isolates (107 to 244 isolates per country) by E test, using Mueller-Hinton agar supplemented with 5% defibrinated horse blood or 5% defibrinated sheep blood, with MICs determined by the National Committee for Clinical Laboratory Standards broth microdilution reference method. Agreement within 1 log2 dilution and minor error rates were 69.3 and 15.5%, respectively, on sheep blood-supplemented agar and 76.9 and 13.6%, respectively, with horse blood as the supplement. Significant interlaboratory variability was observed. E test may not be a reliable method for determining the resistance of pneumococci to trimethoprim-sulfamethoxazole.
一项国际多中心研究，采用补充5%去纤维马血或5%去纤维羊血的穆勒-欣顿琼脂，通过E试验比较了743株肺炎链球菌（每个国家107至244株）对甲氧苄啶-磺胺甲恶唑的最低抑菌浓度（MIC），MIC通过美国国家临床实验室标准委员会肉汤微量稀释参考方法测定。在补充羊血的琼脂上，1个对数2稀释内的一致性和微小错误率分别为69.3%和15.5%，以马血作为补充剂时分别为76.9%和13.6%。观察到显著的实验室间变异性。E试验可能不是确定肺炎球菌对甲氧苄啶-磺胺甲恶唑耐药性的可靠方法。
