通过直接与气液色谱联用的质谱法对全甲基化肽进行分离和测序。
The separation and sequencing of permethylated peptides by mass spectrometry directly coupled to gas-liquid chromatography.
作者信息
Priddle J D, Rose K, Offord R E
出版信息
Biochem J. 1976 Sep 1;157(3):777-80. doi: 10.1042/bj1570777.
Abstract
The use of g.l.c. coupled to mass spectrometry to separate and sequence permethylated acetyl- and trifluoroacetyl-peptides in a single operation is described. Both electron impact and chemical ionization were used to induce fragmentation, and the latter was found to be more sensitive. Chromatographic retention data are presented which suggest that peptide derivatives of molecular weight of at least 750 are accessible to the technique. The application of our methods to the determination of the primary sequence of proteins is discussed.
摘要
本文描述了使用气相色谱-质谱联用技术在一次操作中分离并测序全甲基化的乙酰化和三氟乙酰化肽段。同时使用了电子轰击和化学电离来诱导裂解，结果发现后者更为灵敏。文中给出了色谱保留数据，这些数据表明该技术可用于分子量至少为750的肽衍生物。还讨论了我们的方法在蛋白质一级序列测定中的应用。
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本文引用的文献
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Studies towards the complete sequence determination of proteins by mass spectrometry; a rapid procedure for the successful permethylation of histidine containing peptides.
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