Gupta D K, Ahmad F, Suhail M
Department of Biochemistry & Home Science, Allahabad University, India.
Indian J Exp Biol. 1998 Sep;36(9):934-7.
In order to understand the molecular mechanism of reduced life span of diabetic erythrocyte, polypeptides and glycopeptides were analyzed by disc gel preparative sodium dodecyl sulphate polyacrylamide gel electrophoresis. An additional glycopeptide (244.5 kDa) and two additional polypeptides (39.81 and 144.5 kDa) were observed on glycopeptide and polypeptide gel profiles of mild insulin dependent diabetes mellitus (mIDDM) sample as compared to control. On the basis of molecular weight, their position on gel profile and their widely accepted nomenclature they were termed as glycosylated-ankyrin, membrane accreted glyceraldehyde-3-phosphate dehydrogenease (G 3-PD) and stress induced band 2.3 peptide. Earlier we have reported an increase in heterogeneity associated with increase in the population of aged fragile cells having altered membrane bound cation dependent ATPases, cytosolic dehydrogenase and hexokinase activities of mIDDM simulating rat erythrocyte sample. Significance of above observation in view of our earlier observation is discussed to explain the molecular mechanism of reduced life span of diabetic erythrocytes.
为了了解糖尿病红细胞寿命缩短的分子机制，通过圆盘凝胶制备型十二烷基硫酸钠聚丙烯酰胺凝胶电泳对多肽和糖肽进行了分析。与对照组相比，在轻度胰岛素依赖型糖尿病（mIDDM）样本的糖肽和多肽凝胶图谱上观察到一种额外的糖肽（244.5 kDa）和两种额外的多肽（39.81 kDa和144.5 kDa）。根据分子量、它们在凝胶图谱上的位置以及广泛接受的命名法，它们分别被称为糖基化锚蛋白、膜附着的甘油醛-3-磷酸脱氢酶（G 3-PD）和应激诱导带2.3肽。此前我们报道过，在模拟mIDDM大鼠红细胞样本中，与衰老脆弱细胞群体增加相关的异质性增加，这些细胞具有改变的膜结合阳离子依赖性ATP酶、胞质脱氢酶和己糖激酶活性。鉴于我们之前的观察结果，讨论了上述观察结果的意义，以解释糖尿病红细胞寿命缩短的分子机制。
