Yoshino Tomoyuki, Sugiyama Shigeru, Hagiwara Shoji, Fukushi Daisuke, Shichiri Motoharu, Nakao Hidenobu, Kim Jong-Min, Hirose Tamaki, Muramatsu Hiroshi, Ohtani Toshio
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Ultramicroscopy. 2003 Oct-Nov;97(1-4):81-7. doi: 10.1016/S0304-3991(03)00032-9.
Nano-scale structures of the YOYO-1-stained barley chromosomes and lambda-phage DNA were investigated by scanning near-field optical/atomic force microscopy (SNOM/AFM). This technique enabled precise analysis of fluorescence structural images in relation to the morphology of the biomaterials. The results suggested that the fluorescence intensity does not always correspond to topographic height of the chromosomes, but roughly reflects the local amount and/or density of DNA. Various sizes of the bright fluorescence spots were clearly observed in fluorescence banding-treated chromosomes. Furthermore, fluorescence-stained lambda-phage DNA analysis by SNOM/AFM demonstrated the possibility of nanometer-scale imaging for a novel technique termed nano-fluorescence in situ hybridization (nano-FISH). Thus, SNOM/AFM is a powerful tool for analyzing the structure and the function of biomaterials with higher resolution than conventional optical microscopes.
通过扫描近场光学/原子力显微镜（SNOM/AFM）研究了用YOYO-1染色的大麦染色体和λ噬菌体DNA的纳米级结构。该技术能够精确分析与生物材料形态相关的荧光结构图像。结果表明，荧光强度并不总是与染色体的地形高度相对应，但大致反映了DNA的局部数量和/或密度。在荧光带处理的染色体中清楚地观察到各种大小的明亮荧光斑点。此外，通过SNOM/AFM对荧光染色的λ噬菌体DNA进行分析，证明了一种称为纳米荧光原位杂交（nano-FISH）的新技术进行纳米级成像的可能性。因此，SNOM/AFM是一种比传统光学显微镜具有更高分辨率的分析生物材料结构和功能的强大工具。
