Santhosh A, Lakshmi L R, Arun P, Deepadevi K V, Nair K G, Manojkumar V, Kurup P A
Department of Biological Sciences, Peninsula Polymers Limited, Trivandrum, India.
Indian J Biochem Biophys. 1998 Aug;35(4):236-40.
Synthesis of NAD+ from nicotinic acid by erythrocytes incubated in SAGM phosphate solution and effect of di-[2-ethyl hexyl] phthalate, a plasticizer commonly used in PVC blood/component storage bags, on this synthesis was studied. Erythrocytes are able to synthesise NAD+ in SAGM (sodium chloride, adenine, glucose, mannitol) phosphate solution and this synthesis was more in the presence of added nicotinic acid (optimum concentration 1 mM). The level of NAD+ decreased when the incubation period was increased from 24 to 48 hr. Glutamine had a deleterious effect on this synthesis, possibly due to the decrease in pH. Di-[2-ethyl hexyl] phthalate had an inhibitory effect on NAD+ synthesis when the cells were incubated in SAGM phosphate solution, either alone or in the presence of added nicotinic acid. There was significant decrease in the release of potassium and haemoglobin from the cells in the presence of nicotinic acid, indicating increased red cell stability.
研究了在SAGM磷酸盐溶液中孵育的红细胞由烟酸合成NAD⁺的情况，以及邻苯二甲酸二（2-乙基己基）酯（一种常用于聚氯乙烯血液/成分储存袋的增塑剂）对该合成过程的影响。红细胞能够在SAGM（氯化钠、腺嘌呤、葡萄糖、甘露醇）磷酸盐溶液中合成NAD⁺，在添加烟酸（最佳浓度为1 mM）的情况下合成量更多。当孵育时间从24小时增加到48小时时，NAD⁺水平下降。谷氨酰胺对该合成过程有有害影响，可能是由于pH值降低所致。当细胞在SAGM磷酸盐溶液中单独孵育或在添加烟酸的情况下孵育时，邻苯二甲酸二（2-乙基己基）酯对NAD⁺合成有抑制作用。在存在烟酸的情况下，细胞中钾和血红蛋白的释放显著减少，表明红细胞稳定性增加。
