Mikamo H, Kawazoe K, Sato Y, Imai A, Tamaya T
Department of Obstetrics and Gynecology, School of Medicine, Gifu University, Gifu, Japan.
Am J Obstet Gynecol. 1998 Dec;179(6 Pt 1):1579-82. doi: 10.1016/s0002-9378(98)70028-6.
The studies presented in this report were undertaken to evaluate whether Fusobacterium nucleatum, a common anaerobic isolate in intrauterine infection, stimulates arachidonic acid metabolism, a rate-limiting step for prostaglandin synthesis, in the human uterine endometrium.
Effects of F nucleatum on arachidonic acid liberation from human uterine endometrial cells and of F nucleatum extract on lysophosphatidylcholine production in human uterine endometrial cells were investigated.
When human uterine endometrial cells labeled with tritiated arachidonic acid to an isotopically steady state were exposed to an extract of F nucleatum, arachidonic acid liberation was stimulated, accompanied by lysophospholipid formation. Similar stimulatory effects on phospholipid degradation were also observed in the experiment with bacterially conditioned media.
These results suggest that F nucleatum stimulates endometrial phospholipid metabolism, related to activity of phospholipase A2, which might induce the onset of labor associated with intraamniotic infection.
本报告中的研究旨在评估具核梭杆菌（一种子宫内感染中常见的厌氧分离株）是否会刺激人子宫内膜中花生四烯酸的代谢，这是前列腺素合成的限速步骤。
研究了具核梭杆菌对人子宫内膜细胞释放花生四烯酸的影响以及具核梭杆菌提取物对人子宫内膜细胞中溶血磷脂酰胆碱产生的影响。
当用氚标记的花生四烯酸标记至同位素稳定状态的人子宫内膜细胞暴露于具核梭杆菌提取物时，花生四烯酸的释放受到刺激，并伴有溶血磷脂的形成。在使用细菌条件培养基的实验中也观察到了对磷脂降解的类似刺激作用。
这些结果表明，具核梭杆菌刺激与磷脂酶A2活性相关的子宫内膜磷脂代谢，这可能会引发与羊膜腔内感染相关的分娩。
