Götz M, Stoykova A, Gruss P
Department of Molecular Cell Biology, Max-Planck Institute of Biophysical Chemistry, Göttingen, Federal Republic of Germany.
Neuron. 1998 Nov;21(5):1031-44. doi: 10.1016/s0896-6273(00)80621-2.
Radial glia cells perform a dual function in the developing nervous system as precursor cells and guides for migrating neurons. We show here that during forebrain neurogenesis, the transcription factor Pax6 is specifically localized in radial glia cells of the cortex but not of the basal telencephalon. In Pax6-deficient mice, cortical radial glia cells were altered in their morphology, number, tenascin-C (TN-C) expression, and cell cycle. We show that some of these alterations are cell-autonomous, whereas others were rescued by coculturing with wild-type cortical cells. Our results suggest that Pax6 plays an essential role in the differentiation of cortical radial glia. Thus, despite their widespread distribution, radial glia cells are regionally specified in the developing CNS.
放射状胶质细胞在发育中的神经系统中作为前体细胞和引导迁移神经元的细胞发挥双重功能。我们在此表明，在前脑神经发生过程中，转录因子Pax6特异性地定位于皮质的放射状胶质细胞中，而不是基底前脑的放射状胶质细胞中。在Pax6基因缺陷小鼠中，皮质放射状胶质细胞在形态、数量、腱生蛋白-C（TN-C）表达和细胞周期方面发生了改变。我们表明，其中一些改变是细胞自主性的，而其他改变则通过与野生型皮质细胞共培养得以挽救。我们的结果表明，Pax6在皮质放射状胶质细胞的分化中起重要作用。因此，尽管放射状胶质细胞分布广泛，但在发育中的中枢神经系统中它们是区域特异性的。
