Wu L, Wells D, Tay J, Mendis D, Abbott M A, Barnitt A, Quinlan E, Heynen A, Fallon J R, Richter J D
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA.
Neuron. 1998 Nov;21(5):1129-39. doi: 10.1016/s0896-6273(00)80630-3.
Long-term changes in synaptic efficacy may require the regulated translation of dendritic mRNAs. While the basis of such regulation is unknown, it seemed possible that some features of translational control in development could be recapitulated in neurons. Polyadenylation-induced translation of oocyte mRNAs requires the cis-acting CPE sequence and the CPE-binding protein CPEB. CPEB is also present in the dendritic layers of the hippocampus, at synapses in cultured neurons, and in postsynaptic densities of adult brain. alpha-CaMKII mRNA, which is localized in dendrites and is necessary for synaptic plasticity and LTP, contains two CPEs. These CPEs are bound by CPEB and mediate polyadenylation-induced translation in injected Xenopus oocytes. In the intact brain, visual experience induces alpha-CaMKII mRNA polyadenylation and translation, suggesting that this process likely occurs at synapses.
突触效能的长期变化可能需要对树突状mRNA进行调控翻译。虽然这种调控的基础尚不清楚，但发育过程中翻译控制的某些特征似乎有可能在神经元中重现。卵母细胞mRNA的多聚腺苷酸化诱导翻译需要顺式作用的CPE序列和CPE结合蛋白CPEB。CPEB也存在于海马体的树突层、培养神经元的突触以及成人大脑的突触后致密物中。α-CaMKII mRNA定位于树突中，对突触可塑性和长时程增强是必需的，它包含两个CPE。这些CPE与CPEB结合，并在注射的非洲爪蟾卵母细胞中介导多聚腺苷酸化诱导的翻译。在完整的大脑中，视觉经验会诱导α-CaMKII mRNA的多聚腺苷酸化和翻译，这表明这个过程可能发生在突触处。
