Shahidi M, Zeimer R, Mori M, Blair N
Applied Physics Laboratory, University of Illinois at Chicago Eye Center, USA.
Invest Ophthalmol Vis Sci. 1998 Dec;39(13):2733-43.
To report a new method for optical sectioning of the chorioretinal vasculature to improve the visualization of vascular abnormalities due to chorioretinal eye diseases.
An imaging system was developed for optical sectioning of the vasculature called chorioretinal optical sectioning (CROS). CROS consists of projecting a laser beam at an angle on the retina after injection of a fluorescent dye and viewing the fluorescence. On the fluorescence optical section (FOS) image, the vasculature of the retina and choroid appear laterally displaced according to their depth location. The laser beam is scanned over a 2 X 2-mm area to generate 40 FOS images, each spatially separated by 50 microm on the retina. Optical section images of the vascular layers are constructed from the series of FOS images.
CROS permitted optical separation of vascular layers in living eyes. Optical section images of normal and laser-photocoagulated retinas had higher contrast than conventional angiography because of the separation of the fluorescence from the overlapping layers and allowed enhanced visualization of vascular abnormalities.
CROS enhances the visualization of the retinal and subretinal vasculature and promises to be a beneficial tool for evaluation of chorioretinal diseases.
报告一种用于脉络膜视网膜血管光学切片的新方法，以改善对脉络膜视网膜眼病所致血管异常的可视化。
开发了一种用于血管光学切片的成像系统，称为脉络膜视网膜光学切片（CROS）。CROS包括在注射荧光染料后以一定角度将激光束投射到视网膜上并观察荧光。在荧光光学切片（FOS）图像上，视网膜和脉络膜的血管根据其深度位置出现横向移位。激光束在2×2毫米区域内扫描以生成40张FOS图像，每张图像在视网膜上的空间间隔为50微米。血管层的光学切片图像由一系列FOS图像构建而成。
CROS能够在活体眼中实现血管层的光学分离。正常视网膜和激光光凝视网膜的光学切片图像比传统血管造影具有更高的对比度，这是因为荧光从重叠层中分离出来，并且能够增强对血管异常的可视化。
CROS增强了视网膜和视网膜下血管的可视化，有望成为评估脉络膜视网膜疾病的有益工具。
