Murray M J, Merritt D J, Brand A H, Whitington P M
Division of Molecular and Cellular Biology, School of Biological Sciences, University of New England, Armidale, NSW, Australia.
J Neurobiol. 1998 Dec;37(4):607-21.
We developed a system for time-lapse observation of identified neurons in the central nervous system (CNS) of the Drosophila embryo. Using this system, we characterize the dynamics of filopodia and axon growth of the motorneuron RP2 as it navigates anteriorly through the CNS and then laterally along the intersegmental nerve (ISN) into the periphery. We find that both axonal extension and turning occur primarily through the process of filopodial dilation. In addition, we used the GAL4-UAS system to express the fusion protein Tau-GFP in a subset of neurons, allowing us to correlate RP2's patterns of growth with a subset of axons in its environment. In particular, we show that RP2's sharp lateral turn is coincident with the nascent ISN.
我们开发了一种用于对果蝇胚胎中枢神经系统（CNS）中已识别神经元进行延时观察的系统。利用该系统，我们对运动神经元RP2的丝状伪足和轴突生长动力学进行了表征，该神经元先向前穿过中枢神经系统，然后沿着节间神经（ISN）横向进入外周。我们发现轴突的延伸和转向主要通过丝状伪足扩张的过程发生。此外，我们使用GAL4-UAS系统在一部分神经元中表达融合蛋白Tau-GFP，从而使我们能够将RP2的生长模式与其周围环境中的一部分轴突相关联。特别是，我们表明RP2的急剧横向转向与新生的ISN同时发生。
