Department of Genetics, Microbiology and Statistics, School of Biology, University of Barcelona, Barcelona, Spain.
Institute of Biomedicine University of Barcelona (IBUB), Barcelona, Spain.
Elife. 2020 Oct 28;9:e61111. doi: 10.7554/eLife.61111.
Subcellular lumen formation by single-cells involves complex cytoskeletal remodelling. We have previously shown that centrosomes are key players in the initiation of subcellular lumen formation in , but not much is known on the what leads to the growth of these subcellular luminal branches or makes them progress through a particular trajectory within the cytoplasm. Here, we have identified that the spectraplakin Short-stop (Shot) promotes the crosstalk between MTs and actin, which leads to the extension and guidance of the subcellular lumen within the tracheal terminal cell (TC) cytoplasm. Shot is enriched in cells undergoing the initial steps of subcellular branching as a direct response to FGF signalling. An excess of Shot induces ectopic acentrosomal luminal branching points in the embryonic and larval tracheal TC leading to cells with extra-subcellular lumina. These data provide the first evidence for a role for spectraplakins in single-cell lumen formation and branching.
单细胞的亚细胞腔形成涉及复杂的细胞骨架重塑。我们之前已经表明，中心体是启动 亚细胞腔形成的关键因素，但对于导致这些亚细胞腔分支生长的原因或使它们在细胞质内沿着特定轨迹前进的原因知之甚少。在这里，我们已经确定spectraplakin Short-stop (Shot) 促进了 MTs 和肌动蛋白之间的交流，从而导致了气管末端细胞 (TC) 细胞质内亚细胞腔的延伸和引导。Shot 在细胞经历亚细胞分支的初始步骤时被富集，这是对 FGF 信号的直接反应。Shot 的过量表达会在胚胎和幼虫的气管 TC 中诱导中心体外的腔分支点，导致细胞具有额外的亚细胞腔。这些数据首次提供了 spectraplakins 在单细胞腔形成和分支中的作用的证据。
