Reif A E, Robinson C M
J Immunol. 1976 Aug;117(2):597-601.
Rapid degradation of ascites tumor cells damaged by the action of antibody plus complement was found to be accomplished by all proteolytic enzymes active at physiologic pH that were tested. For three types of murine ascites tumor cells (Ehrlich ascites, sarcoma-180, and L1210 leukemia), this rate of degradation at low trypsin concentrations was proportional to a high power of enzyme concentration. This suggests that the simultaneous action of two or more enzyme molecules at adjacent cell surface sites is necessary. Cell degradation was assayed by determination of cell volume distribution with a Coulter multi-channel particle size analyzer. The present study may offer clues to in vivo mechanisms of cell degradation.
研究发现，抗体加补体作用所损伤的腹水肿瘤细胞的快速降解是由所有在生理pH值下具有活性的蛋白水解酶完成的，这些酶都经过了测试。对于三种类型的小鼠腹水肿瘤细胞（艾氏腹水癌、肉瘤-180和L1210白血病细胞），在低胰蛋白酶浓度下的这种降解速率与酶浓度的高次方成正比。这表明两个或更多酶分子在相邻细胞表面位点的同时作用是必要的。通过使用库尔特多通道粒度分析仪测定细胞体积分布来检测细胞降解情况。本研究可能为细胞降解的体内机制提供线索。
