Kawiak J, Miks B
Folia Histochem Cytochem (Krakow). 1979;17(4):345-52.
The proteinase activity was assayed in the leukemia cells L 1210 and in the ascites fluid with [3H] acetylated haemoglobin as a substrate. The proteinase activity at pH 4.1 increased in cells and in the ascites fluid with age of the tumor. The proteinase activity at pH 7.8 was low, but the enzyme activity in the cell homogenate increased between 5th and 7th day of the tumor growth and it was also present in the ascites fluid. It was observed that the leukemia cells aggregate in vivo and in vitro at pH values of the ascites fluid above pH 7.0. It was suggested, that the aggregation of leukemia cells is due to the tumor cell proteinase activity released to the ascites fluid.
以[3H]乙酰化血红蛋白为底物，在白血病细胞L 1210和腹水中测定蛋白酶活性。随着肿瘤的生长，pH 4.1时细胞和腹水中的蛋白酶活性均升高。pH 7.8时蛋白酶活性较低，但在肿瘤生长的第5天至第7天，细胞匀浆中的酶活性增加，且腹水中也存在该酶活性。观察到白血病细胞在体内和体外，当腹水pH值高于7.0时会聚集。有人认为，白血病细胞的聚集是由于释放到腹水中的肿瘤细胞蛋白酶活性所致。
