骨关节炎和类风湿关节炎滑液中的戊糖苷：与类风湿关节炎疾病活动的关系。

Pentosidine in synovial fluid in osteoarthritis and rheumatoid arthritis: relationship with disease activity in rheumatoid arthritis.

作者信息

Chen J R, Takahashi M, Suzuki M, Kushida K, Miyamoto S, Inoue T

机构信息

Department of Orthopaedic Surgery, Hamamatsu University School of Medicine, Handa, Japan.

出版信息

J Rheumatol. 1998 Dec;25(12):2440-4.

PMID:9858442

Abstract

OBJECTIVE

Pentosidine is an advanced glycation endproduct formed by glycosylation and oxidation. Our aim was to develop a means to measure pentosidine in synovial fluid (SF), and to compare its concentration in SF in patients with osteoarthritis (OA) and rheumatoid arthritis (RA), and to investigate the relationship between its concentration in SF and the disease activity of RA.

METHODS

SF was collected from knee joints in 31 patients with RA and 40 with OA, who had hydrarthrosis. One patient with RA and 7 with OA who had the complication of diabetes mellitus or chronic renal failure made up the DM/CRF group, and the remaining patients made up the RA group (n = 30) and the OA group (n = 33). Pentosidine was measured by the direct HPLC method with column switching after hydrolysis of SF.

RESULTS

Pentosidine was detected in all SF and was greater in RA (83.9 +/- 46.0 nmol/l, mean +/- SD) than in OA (40.1 +/- 19.6 nmol/l). Three DM/CRF patients undergoing hemodialysis had markedly high pentosidine levels (482.5 +/- 280.8 nmol/l). There was a significant correlation between pentosidine and C-reactive protein (CRP), erythrocyte sedimentation rate, and Lansbury Index (p < 0.01). Patients with RA were divided into high and low activity groups according to the CRP and Lansbury Index. Pentosidine was significantly higher in the high activity group (CRP > or = 2.0 mg/dl and Lansbury Index > or = 50%) than in the low activity group (CRP < 2.0 and/or Lansbury Index < 50) (100.9 +/- 42.8 vs 58.5 +/- 39.6 nmol/; p = 0.0013).

CONCLUSION

Pentosidine in synovial fluid was higher in RA than in OA. Pentosidine levels in SF were related to the disease activity in RA.

摘要

目的

戊糖苷是由糖基化和氧化作用形成的晚期糖基化终产物。我们的目的是开发一种测量滑液（SF）中戊糖苷的方法，并比较骨关节炎（OA）和类风湿关节炎（RA）患者滑液中戊糖苷的浓度，以及研究滑液中戊糖苷浓度与RA疾病活动度之间的关系。

方法

从31例患有滑膜炎的RA患者和40例患有滑膜炎的OA患者的膝关节中采集滑液。1例患有糖尿病或慢性肾衰竭并发症的RA患者和7例患有糖尿病或慢性肾衰竭并发症的OA患者组成糖尿病/慢性肾衰竭组，其余患者组成RA组（n = 30）和OA组（n = 33）。滑液水解后，采用柱切换直接高效液相色谱法测定戊糖苷。

结果

在所有滑液中均检测到戊糖苷，RA患者滑液中戊糖苷水平（83.9±46.0 nmol/L，平均值±标准差）高于OA患者（40.1±19.6 nmol/L）。3例接受血液透析的糖尿病/慢性肾衰竭患者的戊糖苷水平明显升高（482.5±280.8 nmol/L）。戊糖苷与C反应蛋白（CRP）、红细胞沉降率和兰斯伯里指数之间存在显著相关性（p < 0.01）。根据CRP和兰斯伯里指数，将RA患者分为高活动度组和低活动度组。高活动度组（CRP≥2.0 mg/dl且兰斯伯里指数≥50%）的戊糖苷水平明显高于低活动度组（CRP < 2.0且/或兰斯伯里指数<50%）（100.9±42.8 vs 58.5±39.6 nmol/；p = 0.0013）。