Wang Z F, Ingledue T C, Dominski Z, Sanchez R, Marzluff W F
Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Mol Cell Biol. 1999 Jan;19(1):835-45. doi: 10.1128/MCB.19.1.835.
Translationally inactive histone mRNA is stored in frog oocytes, and translation is activated at oocyte maturation. The replication-dependent histone mRNAs are not polyadenylated and end in a conserved stem-loop structure. There are two proteins (SLBPs) which bind the 3' end of histone mRNA in frog oocytes. SLBP1 participates in pre-mRNA processing in the nucleus. SLBP2 is oocyte specific, is present in the cytoplasm, and does not support pre-mRNA processing in vivo or in vitro. The stored histone mRNA is bound to SLBP2. As oocytes mature, SLBP2 is degraded and a larger fraction of the histone mRNA is bound to SLBP1. The mechanism of activation of translation of histone mRNAs may involve exchange of SLBPs associated with the 3' end of histone mRNA.
翻译无活性的组蛋白mRNA储存在蛙卵母细胞中，在卵母细胞成熟时翻译被激活。依赖复制的组蛋白mRNA不进行多聚腺苷酸化，而是以保守的茎环结构结尾。在蛙卵母细胞中有两种蛋白（SLBP）与组蛋白mRNA的3'端结合。SLBP1参与细胞核中的前体mRNA加工。SLBP2是卵母细胞特异性的，存在于细胞质中，在体内或体外均不支持前体mRNA加工。储存的组蛋白mRNA与SLBP2结合。随着卵母细胞成熟，SLBP2被降解，大部分组蛋白mRNA与SLBP1结合。组蛋白mRNA翻译激活的机制可能涉及与组蛋白mRNA 3'端相关的SLBP的交换。
