Huang W Q, Zhang C L, Di X Y, Zhang R Q
Department of Histology and Embryology, The Fourth Military Medical University, Xi'an, People's Republic of China.
Placenta. 1998 Nov;19(8):655-61. doi: 10.1016/s0143-4004(98)90027-3.
The localization of 5-hydroxytryptamine (5-HT) and its receptors in human placenta was studied under light and electron microscopy using immunohistochemistry and in situ hybridization. The syncytiotrophoblast, cytotrophoblast, stromal cells and decidual cells in human placentae all appeared to be 5-HT immunoreactive. The 5-HT immunoreactive material was distributed in cytoplasm with negative nuclei. The 5-HT immunoreactive material was also found in capillary endothelium. Trophoblast cells cultured in serum-free medium also showed 5-HT immunoreactivity in the cytoplasm. Fetal white blood cells and both syncytiotrophoblast and cytotrophoblast in human placenta showed 5-HT receptor immunoreactivity and 5-HT1A receptor mRNA hybridized signal was also detected in cytoplasm. The stromal cells and capillary endothelium in placental villi and maternal decidual cells all showed 5-HT receptor immunoreactivity in cytoplasm. The small flattened vesicles and large dense cored vesicles within trophoblast cells showed electron-dense 5-HT receptor immunoreactivity using immunoelectron microscopy. These results suggest that human placenta may not only produce 5-HT but also be a 5-HT target organ, and that 5-HT may not only play roles in placental development and pregnancy maintenance by paracrine and autocrine interactions but may also take part in regulating fetal development.
采用免疫组织化学和原位杂交技术，在光镜和电镜下研究了5-羟色胺（5-HT）及其受体在人胎盘组织中的定位。人胎盘组织中的合体滋养层细胞、细胞滋养层细胞、基质细胞和蜕膜细胞均呈现5-HT免疫反应性。5-HT免疫反应物质分布于细胞质中，细胞核呈阴性。在毛细血管内皮细胞中也发现了5-HT免疫反应物质。在无血清培养基中培养的滋养层细胞在细胞质中也表现出5-HT免疫反应性。人胎盘组织中的胎儿白细胞以及合体滋养层细胞和细胞滋养层细胞均表现出5-HT受体免疫反应性，并且在细胞质中也检测到了5-HT1A受体mRNA杂交信号。胎盘绒毛中的基质细胞和毛细血管内皮细胞以及母体蜕膜细胞在细胞质中均表现出5-HT受体免疫反应性。利用免疫电子显微镜观察发现，滋养层细胞内的小扁平囊泡和大的致密核心囊泡呈现电子致密的5-HT受体免疫反应性。这些结果表明，人胎盘不仅可能产生5-HT，而且可能是5-HT的靶器官，5-HT不仅可能通过旁分泌和自分泌相互作用在胎盘发育和维持妊娠中发挥作用，还可能参与调节胎儿发育。
